1ukf

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{{STRUCTURE_1ukf| PDB=1ukf | SCENE= }}
{{STRUCTURE_1ukf| PDB=1ukf | SCENE= }}
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'''Crystal Structure of Pseudomonas Avirulence Protein AvrPphB'''
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===Crystal Structure of Pseudomonas Avirulence Protein AvrPphB===
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==Overview==
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AvrPphB is an avirulence (Avr) protein from the plant pathogen Pseudomonas syringae that can trigger a disease-resistance response in a number of host plants including Arabidopsis. AvrPphB belongs to a novel family of cysteine proteases with the charter member of this family being the Yersinia effector protein YopT. AvrPphB has a very stringent substrate specificity, catalyzing a single proteolytic cleavage in the Arabidopsis serine/threonine kinase PBS1. We have determined the crystal structure of AvrPphB by x-ray crystallography at 1.35-A resolution. The structure is composed of a central antiparallel beta-sheet, with alpha-helices packing on both sides of the sheet to form a two-lobe structure. The core of this structure resembles the papain-like cysteine proteases. The similarity includes the AvrPphB active site catalytic triad of Cys-98, His-212, and Asp-227 and the oxyanion hole residue Asn-93. Based on analogy with inhibitor complexes of the papain-like proteases, we propose a model for the substrate-binding mechanism of AvrPphB. A deep and positively charged pocket (S2) and a neighboring shallow surface (S3) likely bind to aspartic acid and glycine residues in the substrate located two (P2) and three (P3) residues N terminal to the cleavage site, respectively. Further implications about the specificity of plant pathogen recognition are also discussed.
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(as it appears on PubMed at http://www.pubmed.gov), where 14694194 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14694194}}
==About this Structure==
==About this Structure==
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[[Category: Avrpphb]]
[[Category: Avrpphb]]
[[Category: Hypersensitive response]]
[[Category: Hypersensitive response]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 01:10:26 2008''

Revision as of 22:10, 28 July 2008

Image:1ukf.png

Template:STRUCTURE 1ukf

Crystal Structure of Pseudomonas Avirulence Protein AvrPphB

Template:ABSTRACT PUBMED 14694194

About this Structure

1UKF is a Single protein structure of sequence from Pseudomonas syringae pv. phaseolicola. Full crystallographic information is available from OCA.

Reference

The crystal structure of Pseudomonas avirulence protein AvrPphB: a papain-like fold with a distinct substrate-binding site., Zhu M, Shao F, Innes RW, Dixon JE, Xu Z, Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):302-7. Epub 2003 Dec 23. PMID:14694194

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