1ud3

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{{STRUCTURE_1ud3| PDB=1ud3 | SCENE= }}
{{STRUCTURE_1ud3| PDB=1ud3 | SCENE= }}
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'''Crystal structure of AmyK38 N289H mutant'''
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===Crystal structure of AmyK38 N289H mutant===
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==Overview==
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The crystal structure of a calcium-free alpha-amylase (AmyK38) from Bacillus sp. strain KSM-K38, which resists chelating reagents and chemical oxidants, has been determined by the molecular replacement method and refined to a crystallographic R-factor of 19.9% (R-free of 23.2%) at 2.13-A resolution. The main chain folding of AmyK38 is almost homologous to that of Bacillus licheniformis alpha-amylase. However, neither a highly conserved calcium ion, which is located at the interface between domains A and B, nor any other calcium ions appear to exist in the AmyK38 molecule, although three sodium ions were found, one of which is located at the position corresponding to that of a highly conserved calcium ion of other alpha-amylases. The existence of these sodium ions was crystallographically confirmed by the structures of three metal-exchanged and mutated enzymes. This is the first case in which the structure of the calcium-free alpha-amylase has been determined by crystallography, and it was suggested that these sodium ions, instead of calcium ions, are used to retain the structure and function of AmyK38.
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(as it appears on PubMed at http://www.pubmed.gov), where 12719434 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12719434}}
==About this Structure==
==About this Structure==
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[[Category: Alpha-amylase]]
[[Category: Alpha-amylase]]
[[Category: Calcium-free]]
[[Category: Calcium-free]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:03:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 01:25:26 2008''

Revision as of 22:25, 28 July 2008

Image:1ud3.png

Template:STRUCTURE 1ud3

Crystal structure of AmyK38 N289H mutant

Template:ABSTRACT PUBMED 12719434

About this Structure

1UD3 is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.

Reference

Crystal structure of calcium-free alpha-amylase from Bacillus sp. strain KSM-K38 (AmyK38) and its sodium ion binding sites., Nonaka T, Fujihashi M, Kita A, Hagihara H, Ozaki K, Ito S, Miki K, J Biol Chem. 2003 Jul 4;278(27):24818-24. Epub 2003 Apr 28. PMID:12719434

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