1orq

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Revision as of 21:00, 20 November 2007

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spinqualitylabelsall models 1orq, resolution 3.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

X-ray structure of a voltage-dependent potassium channel in complex with an Fab

Overview

Voltage-dependent K+ channels are members of the family of, voltage-dependent cation (K+, Na+ and Ca2+) channels that open and allow, ion conduction in response to changes in cell membrane voltage. This form, of gating underlies the generation of nerve and muscle action potentials, among other processes. Here we present the structure of KvAP, a, voltage-dependent K+ channel from Aeropyrum pernix. We have determined a, crystal structure of the full-length channel at a resolution of 3.2 A, and, of the isolated voltage-sensor domain at 1.9 A, both in complex with, monoclonal Fab fragments. The channel contains a central ion-conduction, pore surrounded by voltage sensors, which form what we call, 'voltage-sensor paddles'-hydrophobic, cationic, helix-turn-helix, structures on the channel's outer perimeter. Flexible hinges suggest that, the voltage-sensor paddles move in response to membrane voltage changes, carrying their positive charge across the membrane.

About this Structure

1ORQ is a Protein complex structure of sequences from Aeropyrum pernix and Mus musculus with K and CD as ligands. Full crystallographic information is available from OCA.

Reference

X-ray structure of a voltage-dependent K+ channel., Jiang Y, Lee A, Chen J, Ruta V, Cadene M, Chait BT, MacKinnon R, Nature. 2003 May 1;423(6935):33-41. PMID:12721618

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