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1x9m

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[[Image:1x9m.gif|left|200px]]
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{{STRUCTURE_1x9m| PDB=1x9m | SCENE= }}
{{STRUCTURE_1x9m| PDB=1x9m | SCENE= }}
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'''T7 DNA polymerase in complex with an N-2-acetylaminofluorene-adducted DNA'''
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===T7 DNA polymerase in complex with an N-2-acetylaminofluorene-adducted DNA===
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==Overview==
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The carcinogen 2-acetylaminofluorene forms two major DNA adducts: N-(2'-deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) and its deacetylated derivative, N-(2'-deoxyguanosin-8-yl)-2-aminofluorene (dG-AF). Although the dG-AAF and dG-AF adducts are distinguished only by the presence or absence of an acetyl group, they have profoundly different effects on DNA replication. dG-AAF poses a strong block to DNA synthesis and primarily induces frameshift mutations in bacteria, resulting in the loss of one or two nucleotides during replication past the lesion. dG-AF is less toxic and more easily bypassed by DNA polymerases, albeit with an increased frequency of misincorporation opposite the lesion, primarily resulting in G --&gt; T transversions. We present three crystal structures of bacteriophage T7 DNA polymerase replication complexes, one with dG-AAF in the templating position and two others with dG-AF in the templating position. Our crystallographic data suggest why a dG-AAF adduct blocks replication more strongly than does a dG-AF adduct and provide a possible explanation for frameshift mutagenesis during replication bypass of a dG-AAF adduct. The dG-AAF nucleoside adopts a syn conformation that facilitates the intercalation of its fluorene ring into a hydrophobic pocket on the surface of the fingers subdomain and locks the fingers in an open, inactive conformation. In contrast, the dG-AF base at the templating position is not well defined by the electron density, consistent with weak binding to the polymerase and a possible interchange of this adduct between the syn and anti conformations.
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{{ABSTRACT_PUBMED_15528277}}
==About this Structure==
==About this Structure==
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[[Category: N-2-acetylaminofluorene]]
[[Category: N-2-acetylaminofluorene]]
[[Category: Replication block]]
[[Category: Replication block]]
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Revision as of 23:06, 28 July 2008

Image:1x9m.png

Template:STRUCTURE 1x9m

T7 DNA polymerase in complex with an N-2-acetylaminofluorene-adducted DNA

Template:ABSTRACT PUBMED 15528277

About this Structure

1X9M is a Protein complex structure of sequences from Enterobacteria phage t7 and Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structures of 2-acetylaminofluorene and 2-aminofluorene in complex with T7 DNA polymerase reveal mechanisms of mutagenesis., Dutta S, Li Y, Johnson D, Dzantiev L, Richardson CC, Romano LJ, Ellenberger T, Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16186-91. Epub 2004 Nov 4. PMID:15528277

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