This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1osz
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1osz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1osz, resolution 2.1Å" /> '''MHC CLASS I H-2KB HEA...)
Next diff →
Revision as of 21:02, 20 November 2007
|
MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND AN (L4V) MUTANT OF THE VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN
Overview
The T lineage repertoire is shaped by T cell receptor (TCR)-dependent, positive and negative thymic selection processes. Using TCR-transgenic, (N15tg) beta2-microglobulin-deficient (beta2m-/-) RAG-2(-/-) H-2(b) mice, specific for the VSV8 (RGYVYQGL) octapeptide bound to Kb, we identified a, single weak agonist peptide variant V4L (L4) inducing phenotypic and, functional T cell maturation. The cognate VSV8 peptide, in contrast, triggers negative selection. The crystal structure of L4/Kb was determined, and refined to 2.1 A for comparison with the VSV8/Kb structure at similar, resolution. Aside from changes on the p4 side chain of L4 and the, resulting alteration of the exposed Kb Lys-66 side chain, these two, structures are essentially identical. Hence, a given TCR recognizes subtle, distinctions between highly related ligands, resulting in dramatically, different selection outcomes. Based on these finding and the recent, structural elucidation of the N15-VSV8/Kb complex, moreover, it appears, that the germ-line Valpha repertoire contributes in a significant way to, positive selection.
About this Structure
1OSZ is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Differential thymic selection outcomes stimulated by focal structural alteration in peptide/major histocompatibility complex ligands., Ghendler Y, Teng MK, Liu JH, Witte T, Liu J, Kim KS, Kern P, Chang HC, Wang JH, Reinherz EL, Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10061-6. PMID:9707600
Page seeded by OCA on Tue Nov 20 23:10:00 2007
