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1w7c

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[[Image:1w7c.gif|left|200px]]
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{{STRUCTURE_1w7c| PDB=1w7c | SCENE= }}
{{STRUCTURE_1w7c| PDB=1w7c | SCENE= }}
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'''PPLO AT 1.23 ANGSTROMS'''
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===PPLO AT 1.23 ANGSTROMS===
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==Overview==
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The structure of Pichia pastoris lysyl oxidase (PPLO) in a new crystal form has been refined at 1.23 Angstrom resolution. PPLO, a copper amine oxidase (CuAO) with a 2,4,5-trihydroxyphenylalanine quinone (TPQ) cofactor, differs from most other members of the CuAO enzyme family in having the ability to oxidize the side chain of lysine residues in a polypeptide. In the asymmetric unit of the crystals, the structure analysis has located residues 43-779 of the polypeptide chain, seven carbohydrate residues, the active-site Cu atom, an imidazole molecule bound at the active site, two buried Ca(2+) ions, five surface Mg(2+) ions, five surface Cl(-) ions and 1045 water molecules. The crystallographic residuals are R = 0.112 and R(free) = 0.146. The TPQ cofactor and several other active-site residues are poorly ordered, in contrast to the surrounding well ordered structure. A covalent cross-link is observed between two lysine residues, Lys778 and Lys66. The cross-link is likely to have been formed by the oxidation of Lys778 followed by a spontaneous reaction with Lys66. The link is modelled as dehydrolysinonorleucine.
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(as it appears on PubMed at http://www.pubmed.gov), where 16929109 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16929109}}
==About this Structure==
==About this Structure==
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[[Category: Topaquinone enzyme]]
[[Category: Topaquinone enzyme]]
[[Category: Tpq]]
[[Category: Tpq]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:15:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:15:32 2008''

Revision as of 23:15, 28 July 2008

Image:1w7c.png

Template:STRUCTURE 1w7c

PPLO AT 1.23 ANGSTROMS

Template:ABSTRACT PUBMED 16929109

About this Structure

1W7C is a Single protein structure of sequence from Pichia pastoris. Full crystallographic information is available from OCA.

Reference

The 1.23 Angstrom structure of Pichia pastoris lysyl oxidase reveals a lysine-lysine cross-link., Duff AP, Cohen AE, Ellis PJ, Hilmer K, Langley DB, Dooley DM, Freeman HC, Guss JM, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1073-84. Epub 2006, Aug 19. PMID:16929109

Page seeded by OCA on Tue Jul 29 02:15:32 2008

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