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3cfb

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[[Image:3cfb.jpg|left|200px]]
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{{STRUCTURE_3cfb| PDB=3cfb | SCENE= }}
{{STRUCTURE_3cfb| PDB=3cfb | SCENE= }}
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'''High-resolution structure of blue fluorescent antibody EP2-19G2 in complex with stilbene hapten at 100K'''
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===High-resolution structure of blue fluorescent antibody EP2-19G2 in complex with stilbene hapten at 100K===
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==Overview==
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The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel pi-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.
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(as it appears on PubMed at http://www.pubmed.gov), where 18309081 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18309081}}
==About this Structure==
==About this Structure==
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[[Category: Immune system]]
[[Category: Immune system]]
[[Category: Immunoglobulin]]
[[Category: Immunoglobulin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 21:42:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:22:29 2008''

Revision as of 23:22, 28 July 2008

Image:3cfb.png

Template:STRUCTURE 3cfb

High-resolution structure of blue fluorescent antibody EP2-19G2 in complex with stilbene hapten at 100K

Template:ABSTRACT PUBMED 18309081

About this Structure

3CFB is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex., Debler EW, Kaufmann GF, Meijler MM, Heine A, Mee JM, Pljevaljcic G, Di Bilio AJ, Schultz PG, Millar DP, Janda KD, Wilson IA, Gray HB, Lerner RA, Science. 2008 Feb 29;319(5867):1232-5. PMID:18309081

Page seeded by OCA on Tue Jul 29 02:22:29 2008

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