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1yyg

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{{STRUCTURE_1yyg| PDB=1yyg | SCENE= }}
{{STRUCTURE_1yyg| PDB=1yyg | SCENE= }}
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'''Manganese peroxidase complexed with Cd(II) inhibitor'''
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===Manganese peroxidase complexed with Cd(II) inhibitor===
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==Overview==
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Manganese peroxidase (MnP) is an extracellular heme enzyme that catalyzes the peroxide-dependent oxidation of Mn(II) to Mn(III). The Mn(III) is released from the enzyme in complex with oxalate. One heme propionate and the side chains of Glu35, Glu39, and Asp179 were identified as Mn(II) ligands in the 2.0 A resolution crystal structure. The new 1.45 A crystal structure of MnP complexed with Mn(II) provides a more accurate view of the Mn-binding site. New features include possible partial protonation of Glu39 in the Mn-binding site and glycosylation at Ser336. This is also the first report of MnP-inhibitor complex structures. At the Mn-binding site, divalent Cd(II) exhibits octahedral, hexacoordinate ligation geometry similar to that of Mn(II). Cd(II) also binds to a putative second weak metal-binding site with tetrahedral geometry at the C-terminus of the protein. Unlike that for Mn(II) and Cd(II), coordination of trivalent Sm(III) at the Mn-binding site is octacoordinate. Sm(III) was removed from a MnP-Sm(III) crystal by soaking the crystal in oxalate and then reintroduced into the binding site. Thus, direct comparisons of Sm(III)-bound and metal-free structures were made using the same crystal. No ternary complex was observed upon incubation with oxalate. The reversible binding of Sm(III) may be a useful model for the reversible binding of Mn(III) to the enzyme, which is too unstable to allow similar examination.
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(as it appears on PubMed at http://www.pubmed.gov), where 15850380 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15850380}}
==About this Structure==
==About this Structure==
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[[Category: Heme enzyme]]
[[Category: Heme enzyme]]
[[Category: Peroxidase]]
[[Category: Peroxidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:57:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:27:59 2008''

Revision as of 23:28, 28 July 2008

Image:1yyg.png

Template:STRUCTURE 1yyg

Manganese peroxidase complexed with Cd(II) inhibitor

Template:ABSTRACT PUBMED 15850380

About this Structure

1YYG is a Single protein structure of sequence from Phanerochaete chrysosporium. Full crystallographic information is available from OCA.

Reference

High-resolution crystal structure of manganese peroxidase: substrate and inhibitor complexes., Sundaramoorthy M, Youngs HL, Gold MH, Poulos TL, Biochemistry. 2005 May 3;44(17):6463-70. PMID:15850380

Page seeded by OCA on Tue Jul 29 02:27:59 2008

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