1zc3

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1zc3.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1zc3.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1zc3| PDB=1zc3 | SCENE= }}
{{STRUCTURE_1zc3| PDB=1zc3 | SCENE= }}
-
'''Crystal structure of the Ral-binding domain of Exo84 in complex with the active RalA'''
+
===Crystal structure of the Ral-binding domain of Exo84 in complex with the active RalA===
-
==Overview==
+
<!--
-
The Sec6/8 complex, also known as the exocyst complex, is an octameric protein complex that has been implicated in tethering of secretory vesicles to specific regions on the plasma membrane. Two subunits of the Sec6/8 complex, Exo84 and Sec5, have recently been shown to be effector targets for active Ral GTPases. However, the mechanism by which Ral proteins regulate the Sec6/8 activities remains unclear. Here, we present the crystal structure of the Ral-binding domain of Exo84 in complex with active RalA. The structure reveals that the Exo84 Ral-binding domain adopts a pleckstrin homology domain fold, and that RalA interacts with Exo84 via an extended interface that includes both switch regions. Key residues of Exo84 and RalA were found that determine the specificity of the complex interactions; these interactions were confirmed by mutagenesis binding studies. Structural and biochemical data show that Exo84 and Sec5 competitively bind to active RalA. Taken together, these results further strengthen the proposed role of RalA-regulated assembly of the Sec6/8 complex.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15920473}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15920473 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15920473}}
==About this Structure==
==About this Structure==
Line 32: Line 36:
[[Category: Gtp-binding protein]]
[[Category: Gtp-binding protein]]
[[Category: Small gtpase]]
[[Category: Small gtpase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:26:44 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:28:55 2008''

Revision as of 23:28, 28 July 2008

Image:1zc3.png

Template:STRUCTURE 1zc3

Crystal structure of the Ral-binding domain of Exo84 in complex with the active RalA

Template:ABSTRACT PUBMED 15920473

About this Structure

1ZC3 is a Protein complex structure of sequences from Homo sapiens and Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Exo84 and Sec5 are competitive regulatory Sec6/8 effectors to the RalA GTPase., Jin R, Junutula JR, Matern HT, Ervin KE, Scheller RH, Brunger AT, EMBO J. 2005 Jun 15;24(12):2064-74. Epub 2005 May 26. PMID:15920473

Page seeded by OCA on Tue Jul 29 02:28:55 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1zc3"
Personal tools