1qhw

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[[Image:1qhw.jpg|left|200px]]
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{{Seed}}
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[[Image:1qhw.png|left|200px]]
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{{STRUCTURE_1qhw| PDB=1qhw | SCENE= }}
{{STRUCTURE_1qhw| PDB=1qhw | SCENE= }}
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'''PURPLE ACID PHOSPHATASE FROM RAT BONE'''
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===PURPLE ACID PHOSPHATASE FROM RAT BONE===
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==Overview==
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The crystal structure of purple acid phosphatase from rat bone has been determined by molecular replacement and the structure has been refined to 2.2 A resolution to an R -factor of 21.3 % (R -free 26.5 %). The core of the enzyme consists of two seven-stranded mixed beta-sheets, with each sheet flanked by solvent-exposed alpha-helices on one side. The two sheets pack towards each other forming a beta-sandwich. The di-iron center, located at the bottom of the active-site pocket at one edge of the beta-sandwich, contains a mu-hydroxo or mu-oxo bridge and both metal ions are observed in an almost perfect octahedral coordination geometry. The electron density map indicates that a mu-(hydr)oxo bridge is found in the metal center and that at least one solvent molecule is located in the first coordination sphere of one of the metal ions. The crystallographic study of rat purple acid phosphatase reveals that the mammalian enzymes are very similar in overall structure to the plant enzymes in spite of only 18 % overall sequence identity. In particular, coordination and geometry of the iron cluster is preserved in both enzymes and comparison of the active-sites suggests a common mechanism for the mammalian and plant enzymes. However, significant differences are found in the architecture of the substrate binding pocket.
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(as it appears on PubMed at http://www.pubmed.gov), where 10438611 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10438611}}
==About this Structure==
==About this Structure==
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[[Category: Vihko, P.]]
[[Category: Vihko, P.]]
[[Category: Metal phosphatase]]
[[Category: Metal phosphatase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:17:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:29:18 2008''

Revision as of 23:29, 28 July 2008

Image:1qhw.png

Template:STRUCTURE 1qhw

PURPLE ACID PHOSPHATASE FROM RAT BONE

Template:ABSTRACT PUBMED 10438611

About this Structure

1QHW is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of a mammalian purple acid phosphatase at 2.2 A resolution with a mu-(hydr)oxo bridged di-iron center., Lindqvist Y, Johansson E, Kaija H, Vihko P, Schneider G, J Mol Biol. 1999 Aug 6;291(1):135-47. PMID:10438611

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