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2ign

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[[Image:2ign.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_2ign| PDB=2ign | SCENE= }}
{{STRUCTURE_2ign| PDB=2ign | SCENE= }}
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'''Crystal structure of recombinant pyranose 2-oxidase H167A mutant'''
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===Crystal structure of recombinant pyranose 2-oxidase H167A mutant===
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==Overview==
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Pyranose 2-oxidase (P2Ox) participates in fungal lignin degradation by producing the H2O2 needed for lignin-degrading peroxidases. The enzyme oxidizes cellulose- and hemicellulose-derived aldopyranoses at C2 preferentially, but also on C3, to the corresponding ketoaldoses. To investigate the structural determinants of catalysis, covalent flavinylation, substrate binding, and regioselectivity, wild-type and mutant P2Ox enzymes were produced and characterized biochemically and structurally. Removal of the histidyl-FAD linkage resulted in a catalytically competent enzyme containing tightly, but noncovalently bound FAD. This mutant (H167A) is characterized by a 5-fold lower kcat, and a 35-mV lower redox potential, although no significant structural changes were seen in its crystal structure. In previous structures of P2Ox, the substrate loop (residues 452-457) covering the active site has been either disordered or in a conformation incompatible with carbohydrate binding. We present here the crystal structure of H167A in complex with a slow substrate, 2-fluoro-2-deoxy-D-glucose. Based on the details of 2-fluoro-2-deoxy-D-glucose binding in position for oxidation at C3, we also outline a probable binding mode for D-glucose positioned for regioselective oxidation at C2. The tentative determinant for discriminating between the two binding modes is the position of the O6 hydroxyl group, which in the C2-oxidation mode can make favorable interactions with Asp452 in the substrate loop and, possibly, a nearby arginine residue (Arg472). We also substantiate our hypothesis with steady-state kinetics data for the alanine replacements of Asp452 and Arg472 as well as the double alanine 452/472 mutant.
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(as it appears on PubMed at http://www.pubmed.gov), where 16984920 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16984920}}
==About this Structure==
==About this Structure==
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[[Category: Phbh fold]]
[[Category: Phbh fold]]
[[Category: Rossman fold]]
[[Category: Rossman fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:28:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:34:15 2008''

Revision as of 23:34, 28 July 2008

Image:2ign.png

Template:STRUCTURE 2ign

Crystal structure of recombinant pyranose 2-oxidase H167A mutant

Template:ABSTRACT PUBMED 16984920

About this Structure

2IGN is a Single protein structure of sequence from Trametes ochracea. Full crystallographic information is available from OCA.

Reference

Structural basis for substrate binding and regioselective oxidation of monosaccharides at C3 by pyranose 2-oxidase., Kujawa M, Ebner H, Leitner C, Hallberg BM, Prongjit M, Sucharitakul J, Ludwig R, Rudsander U, Peterbauer C, Chaiyen P, Haltrich D, Divne C, J Biol Chem. 2006 Nov 17;281(46):35104-15. Epub 2006 Sep 19. PMID:16984920

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