2bny

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[[Image:2bny.gif|left|200px]]
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{{STRUCTURE_2bny| PDB=2bny | SCENE= }}
{{STRUCTURE_2bny| PDB=2bny | SCENE= }}
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'''MS2 (N87A MUTANT)- RNA HAIRPIN COMPLEX'''
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===MS2 (N87A MUTANT)- RNA HAIRPIN COMPLEX===
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==Overview==
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Sequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism.
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(as it appears on PubMed at http://www.pubmed.gov), where 16531233 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16531233}}
==About this Structure==
==About this Structure==
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[[Category: Levivirus]]
[[Category: Levivirus]]
[[Category: Virus/viral protein/rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:46:07 2008''

Revision as of 23:46, 28 July 2008

Image:2bny.png

Template:STRUCTURE 2bny

MS2 (N87A MUTANT)- RNA HAIRPIN COMPLEX

Template:ABSTRACT PUBMED 16531233

About this Structure

2BNY is a Protein complex structure of sequences from Enterobacterio phage ms2. Full crystallographic information is available from OCA.

Reference

Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2., Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SE, Stonehouse NJ, Liljas L, Stockley PG, Structure. 2006 Mar;14(3):487-95. PMID:16531233

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