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2c4i

From Proteopedia

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{{STRUCTURE_2c4i| PDB=2c4i | SCENE= }}
{{STRUCTURE_2c4i| PDB=2c4i | SCENE= }}
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'''CRYSTAL STRUCTURE OF ENGINEERED AVIDIN'''
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===CRYSTAL STRUCTURE OF ENGINEERED AVIDIN===
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==Overview==
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Dual chain avidin (dcAvd) is an engineered avidin form, in which two circularly permuted chicken avidin monomers are fused into one polypeptide chain. DcAvd can theoretically form two different pseudotetrameric quaternary assemblies because of symmetry at the monomer-monomer interfaces. Here, our aim was to control the assembly of the quaternary structure of dcAvd. We introduced the mutation I117C into one of the circularly permuted domains of dcAvd and scanned residues along the 1-3 subunit interface of the other domain. Interestingly, V115H resulted in a single, disulfide locked quaternary assembly of dcAvd, whereas I117H could not guide the oligomerisation process even though it stabilised the protein. The modified dcAvd forms were found to retain their characteristic pseudotetrameric state both at high and low pH, and were shown to bind D-biotin at levels comparable to that of wild-type chicken avidin. The crystal structure of dcAvd-biotin complex at 1.95 Angstroms resolution demonstrates the formation of the functional dcAvd pseudotetramer at the atomic level and reveals the molecular basis for its special properties. Altogether, our data facilitate further engineering of the biotechnologically valuable dcAvd scaffold and gives insights into how to guide the quaternary structure assembly of oligomeric proteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 16787776 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16787776}}
==About this Structure==
==About this Structure==
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[[Category: Glycoprotein]]
[[Category: Glycoprotein]]
[[Category: Signalor]]
[[Category: Signalor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:14:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:50:07 2008''

Revision as of 23:50, 28 July 2008

Image:2c4i.png

Template:STRUCTURE 2c4i

CRYSTAL STRUCTURE OF ENGINEERED AVIDIN

Template:ABSTRACT PUBMED 16787776

About this Structure

2C4I is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

Controlling quaternary structure assembly: subunit interface engineering and crystal structure of dual chain avidin., Hytonen VP, Horha J, Airenne TT, Niskanen EA, Helttunen KJ, Johnson MS, Salminen TA, Kulomaa MS, Nordlund HR, J Mol Biol. 2006 Jun 23;359(5):1352-63. Epub 2006 May 6. PMID:16787776

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