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1u7j

From Proteopedia

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[[Image:1u7j.gif|left|200px]]
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{{STRUCTURE_1u7j| PDB=1u7j | SCENE= }}
{{STRUCTURE_1u7j| PDB=1u7j | SCENE= }}
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'''Solution structure of a diiron protein model'''
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===Solution structure of a diiron protein model===
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==Overview==
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Although the analysis and design of turns that connect the strands in antiparallel beta-hairpins has reached an advanced state, much less is known concerning turns between antiparallel helices in helical hairpins. We have conducted an analysis of the structures and sequence preferences of two types of interhelical turns, each of which connects the two helices by a two-residue linker in an alphaL-beta conformation. Based on this analysis, it became apparent that the turn introduced into a designed four-helix bundle protein, DF1, did not occur within an optimal structural context. DF1 is a dimeric model for the diiron class of proteins. A longer loop with a beta-alphaR-beta conformation was inserted between two helices in the protein, and a sequence was chosen to stabilize its conformation. X-ray crystallography and NMR analysis of the protein showed the structure to be in excellent agreement with design.
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The line below this paragraph, {{ABSTRACT_PUBMED_15713492}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15713492 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15713492}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U7J OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U7J OCA].
==Reference==
==Reference==
Analysis and design of turns in alpha-helical hairpins., Lahr SJ, Engel DE, Stayrook SE, Maglio O, North B, Geremia S, Lombardi A, DeGrado WF, J Mol Biol. 2005 Mar 11;346(5):1441-54. Epub 2005 Jan 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15713492 15713492]
Analysis and design of turns in alpha-helical hairpins., Lahr SJ, Engel DE, Stayrook SE, Maglio O, North B, Geremia S, Lombardi A, DeGrado WF, J Mol Biol. 2005 Mar 11;346(5):1441-54. Epub 2005 Jan 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15713492 15713492]
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Proton and metal ion-dependent assembly of a model diiron protein., Pasternak A, Kaplan J, Lear JD, Degrado WF, Protein Sci. 2001 May;10(5):958-69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11316876 11316876]
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Preorganization of molecular binding sites in designed diiron proteins., Maglio O, Nastri F, Pavone V, Lombardi A, DeGrado WF, Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3772-7. Epub 2003 Mar 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12655072 12655072]
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Inaugural article: retrostructural analysis of metalloproteins: application to the design of a minimal model for diiron proteins., Lombardi A, Summa CM, Geremia S, Randaccio L, Pavone V, DeGrado WF, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6298-305. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10841536 10841536]
[[Category: Calhoun, J R.]]
[[Category: Calhoun, J R.]]
[[Category: DeGrado, W F.]]
[[Category: DeGrado, W F.]]
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[[Category: Inter-helical loop]]
[[Category: Inter-helical loop]]
[[Category: Protein design]]
[[Category: Protein design]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:51:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:54:49 2008''

Revision as of 23:54, 28 July 2008

Image:1u7j.png

Template:STRUCTURE 1u7j

Solution structure of a diiron protein model

Template:ABSTRACT PUBMED 15713492

About this Structure

Full experimental information is available from OCA.

Reference

Analysis and design of turns in alpha-helical hairpins., Lahr SJ, Engel DE, Stayrook SE, Maglio O, North B, Geremia S, Lombardi A, DeGrado WF, J Mol Biol. 2005 Mar 11;346(5):1441-54. Epub 2005 Jan 13. PMID:15713492

Proton and metal ion-dependent assembly of a model diiron protein., Pasternak A, Kaplan J, Lear JD, Degrado WF, Protein Sci. 2001 May;10(5):958-69. PMID:11316876

Preorganization of molecular binding sites in designed diiron proteins., Maglio O, Nastri F, Pavone V, Lombardi A, DeGrado WF, Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3772-7. Epub 2003 Mar 24. PMID:12655072

Inaugural article: retrostructural analysis of metalloproteins: application to the design of a minimal model for diiron proteins., Lombardi A, Summa CM, Geremia S, Randaccio L, Pavone V, DeGrado WF, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6298-305. PMID:10841536

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