1owg

From Proteopedia

Revision as of 21:07, 20 November 2007

Crystal structure of WT IHF complexed with an altered H' site (T44A)

Overview

Integration host factor (IHF) is a DNA-bending protein that recognizes its, cognate sites through indirect readout. Previous studies have shown that, binding of wild-type (WT)-IHF is disrupted by a T to A mutation at the, center position of a conserved TTR motif in its binding site, and that, substitution of betaGlu44 with Ala prevented IHF from discriminating, between A and T at this position. We have determined the crystal, structures and relative binding affinities for all combinations of WT-IHF, and IHF-betaGlu44Ala bound to the WT and mutant DNAs. Comparison of these, structures reveals that DNA twist plays a major role in DNA recognition by, IHF, and that this geometric parameter is dependent on the dinucleotide, step and not on the bound IHF variant.

About this Structure

1OWG is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Integration host factor: putting a twist on protein-DNA recognition., Lynch TW, Read EK, Mattis AN, Gardner JF, Rice PA, J Mol Biol. 2003 Jul 11;330(3):493-502. PMID:12842466

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