This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1owg
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1owg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1owg, resolution 2.10Å" /> '''Crystal structure of...)
Next diff →
Revision as of 21:07, 20 November 2007
|
Crystal structure of WT IHF complexed with an altered H' site (T44A)
Overview
Integration host factor (IHF) is a DNA-bending protein that recognizes its, cognate sites through indirect readout. Previous studies have shown that, binding of wild-type (WT)-IHF is disrupted by a T to A mutation at the, center position of a conserved TTR motif in its binding site, and that, substitution of betaGlu44 with Ala prevented IHF from discriminating, between A and T at this position. We have determined the crystal, structures and relative binding affinities for all combinations of WT-IHF, and IHF-betaGlu44Ala bound to the WT and mutant DNAs. Comparison of these, structures reveals that DNA twist plays a major role in DNA recognition by, IHF, and that this geometric parameter is dependent on the dinucleotide, step and not on the bound IHF variant.
About this Structure
1OWG is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Integration host factor: putting a twist on protein-DNA recognition., Lynch TW, Read EK, Mattis AN, Gardner JF, Rice PA, J Mol Biol. 2003 Jul 11;330(3):493-502. PMID:12842466
Page seeded by OCA on Tue Nov 20 23:14:59 2007
