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1srk

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[[Image:1srk.gif|left|200px]]
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{{STRUCTURE_1srk| PDB=1srk | SCENE= }}
{{STRUCTURE_1srk| PDB=1srk | SCENE= }}
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'''Solution structure of the third zinc finger domain of FOG-1'''
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===Solution structure of the third zinc finger domain of FOG-1===
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==Overview==
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Classic zinc finger domains (cZFs) consist of a beta-hairpin followed by an alpha-helix. They are among the most abundant of all protein domains and are often found in tandem arrays in DNA-binding proteins, with each finger contributing an alpha-helix to effect sequence-specific DNA recognition. Lone cZFs, not found in tandem arrays, have been postulated to function in protein interactions. We have studied the transcriptional co-regulator Friend of GATA (FOG), which contains nine zinc fingers. We have discovered that the third cZF of FOG contacts a coiled-coil domain in the centrosomal protein transforming acidic coiled-coil 3 (TACC3). Although FOG-ZF3 exhibited low solubility, we have used a combination of mutational mapping and protein engineering to generate a derivative that was suitable for in vitro and structural analysis. We report that the alpha-helix of FOG-ZF3 recognizes a C-terminal portion of the TACC3 coiled-coil. Remarkably, the alpha-helical surface utilized by FOG-ZF3 is the same surface responsible for the well established sequence-specific DNA-binding properties of many other cZFs. Our data demonstrate the versatility of cZFs and have implications for the analysis of many as yet uncharacterized cZF proteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 15234987 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15234987}}
==About this Structure==
==About this Structure==
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1SRK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SRK OCA].
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1SRK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SRK OCA].
==Reference==
==Reference==
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[[Category: Simpson, R J.Y.]]
[[Category: Simpson, R J.Y.]]
[[Category: Classical zinc finger]]
[[Category: Classical zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:03:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:07:49 2008''

Revision as of 00:07, 29 July 2008

Image:1srk.png

Template:STRUCTURE 1srk

Solution structure of the third zinc finger domain of FOG-1

Template:ABSTRACT PUBMED 15234987

About this Structure

1SRK is a Single protein structure of sequence from Mus musculus. Full experimental information is available from OCA.

Reference

A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3., Simpson RJ, Yi Lee SH, Bartle N, Sum EY, Visvader JE, Matthews JM, Mackay JP, Crossley M, J Biol Chem. 2004 Sep 17;279(38):39789-97. Epub 2004 Jul 2. PMID:15234987

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