1srk

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1srk.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1srk.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1srk| PDB=1srk | SCENE= }}
{{STRUCTURE_1srk| PDB=1srk | SCENE= }}
-
'''Solution structure of the third zinc finger domain of FOG-1'''
+
===Solution structure of the third zinc finger domain of FOG-1===
-
==Overview==
+
<!--
-
Classic zinc finger domains (cZFs) consist of a beta-hairpin followed by an alpha-helix. They are among the most abundant of all protein domains and are often found in tandem arrays in DNA-binding proteins, with each finger contributing an alpha-helix to effect sequence-specific DNA recognition. Lone cZFs, not found in tandem arrays, have been postulated to function in protein interactions. We have studied the transcriptional co-regulator Friend of GATA (FOG), which contains nine zinc fingers. We have discovered that the third cZF of FOG contacts a coiled-coil domain in the centrosomal protein transforming acidic coiled-coil 3 (TACC3). Although FOG-ZF3 exhibited low solubility, we have used a combination of mutational mapping and protein engineering to generate a derivative that was suitable for in vitro and structural analysis. We report that the alpha-helix of FOG-ZF3 recognizes a C-terminal portion of the TACC3 coiled-coil. Remarkably, the alpha-helical surface utilized by FOG-ZF3 is the same surface responsible for the well established sequence-specific DNA-binding properties of many other cZFs. Our data demonstrate the versatility of cZFs and have implications for the analysis of many as yet uncharacterized cZF proteins.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15234987}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15234987 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15234987}}
==About this Structure==
==About this Structure==
-
1SRK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SRK OCA].
+
1SRK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SRK OCA].
==Reference==
==Reference==
Line 29: Line 33:
[[Category: Simpson, R J.Y.]]
[[Category: Simpson, R J.Y.]]
[[Category: Classical zinc finger]]
[[Category: Classical zinc finger]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:03:49 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:07:49 2008''

Revision as of 00:07, 29 July 2008

Image:1srk.png

Template:STRUCTURE 1srk

Solution structure of the third zinc finger domain of FOG-1

Template:ABSTRACT PUBMED 15234987

About this Structure

1SRK is a Single protein structure of sequence from Mus musculus. Full experimental information is available from OCA.

Reference

A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3., Simpson RJ, Yi Lee SH, Bartle N, Sum EY, Visvader JE, Matthews JM, Mackay JP, Crossley M, J Biol Chem. 2004 Sep 17;279(38):39789-97. Epub 2004 Jul 2. PMID:15234987

Page seeded by OCA on Tue Jul 29 03:07:49 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1srk"
Personal tools