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2du8

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{{STRUCTURE_2du8| PDB=2du8 | SCENE= }}
{{STRUCTURE_2du8| PDB=2du8 | SCENE= }}
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'''Crystal structure of human D-amino acid oxidase'''
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===Crystal structure of human D-amino acid oxidase===
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==Overview==
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In the brain, the extensively studied FAD-dependent enzyme D-amino acid oxidase (DAO) degrades the gliotransmitter D-serine, a potent activator of N-methyl-D-aspartate type glutamate receptors, and evidence suggests that DAO, together with its activator G72 protein, may play a key role in the pathophysiology of schizophrenia. Indeed, its potential clinical importance highlights the need for structural and functional analyses of human DAO. We recently succeeded in purifying human DAO, and found that it weakly binds FAD and shows a significant slower rate of flavin reduction compared with porcine DAO. However, the molecular basis for the different kinetic features remains unclear because the active site of human DAO was considered to be virtually identical to that of porcine DAO, as would be expected from the 85% sequence identity. To address this issue, we determined the crystal structure of human DAO in complex with a competitive inhibitor benzoate, at a resolution of 2.5 Angstrom. The overall dimeric structure of human DAO is similar to porcine DAO, and the catalytic residues are fully conserved at the re-face of the flavin ring. However, at the si-face of the flavin ring, despite the strict sequence identity, a hydrophobic stretch (residues 47-51, VAAGL) exists in a significantly different conformation compared with both of the independently determined porcine DAO-benzoate structures. This suggests that a context-dependent conformational variability of the hydrophobic stretch accounts for the low affinity for FAD as well as the slower rate of flavin reduction, thus highlighting the unique features of the human enzyme.
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(as it appears on PubMed at http://www.pubmed.gov), where 17088322 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17088322}}
==About this Structure==
==About this Structure==
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[[Category: Conformational variability]]
[[Category: Conformational variability]]
[[Category: Structurally ambivalent peptide]]
[[Category: Structurally ambivalent peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 01:23:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:17:57 2008''

Revision as of 00:17, 29 July 2008

Image:2du8.png

Template:STRUCTURE 2du8

Crystal structure of human D-amino acid oxidase

Template:ABSTRACT PUBMED 17088322

About this Structure

2DU8 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human D-amino acid oxidase: context-dependent variability of the backbone conformation of the VAAGL hydrophobic stretch located at the si-face of the flavin ring., Kawazoe T, Tsuge H, Pilone MS, Fukui K, Protein Sci. 2006 Dec;15(12):2708-17. Epub 2006 Nov 6. PMID:17088322

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