2f52

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{{STRUCTURE_2f52| PDB=2f52 | SCENE= }}
{{STRUCTURE_2f52| PDB=2f52 | SCENE= }}
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'''Solution structure of cold shock protein CspB from Bacillus subtilis in complex with heptathymidine'''
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===Solution structure of cold shock protein CspB from Bacillus subtilis in complex with heptathymidine===
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==Overview==
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Cold shock proteins (CSP) belong to the family of single-stranded nucleic acid binding proteins with OB-fold. CSP are believed to function as 'RNA chaperones' and during anti-termination. We determined the solution structure of Bs-CspB bound to the single-stranded DNA (ssDNA) fragment heptathymidine (dT7) by NMR spectroscopy. Bs-CspB reveals an almost invariant conformation when bound to dT7 with only minor reorientations in loop beta1-beta2 and beta3-beta4 and of few aromatic side chains involved in base stacking. Binding studies of protein variants and mutated ssDNA demonstrated that Bs-CspB associates with ssDNA at almost diffusion controlled rates and low sequence specificity consistent with its biological function. A variation of the ssDNA affinity is accomplished solely by changes of the dissociation rate. 15N NMR relaxation and H/D exchange experiments revealed that binding of dT7 increases the stability of Bs-CspB and reduces the sub-nanosecond dynamics of the entire protein and especially of loop beta3-beta4.
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{{ABSTRACT_PUBMED_16956971}}
==About this Structure==
==About this Structure==
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2F52 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F52 OCA].
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2F52 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F52 OCA].
==Reference==
==Reference==
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[[Category: Beta barrel]]
[[Category: Beta barrel]]
[[Category: Ob-fold]]
[[Category: Ob-fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:28:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:22:27 2008''

Revision as of 00:22, 29 July 2008

Image:2f52.png

Template:STRUCTURE 2f52

Solution structure of cold shock protein CspB from Bacillus subtilis in complex with heptathymidine

Template:ABSTRACT PUBMED 16956971

About this Structure

2F52 is a Single protein structure of sequence from Bacillus subtilis. Full experimental information is available from OCA.

Reference

Recognition of T-rich single-stranded DNA by the cold shock protein Bs-CspB in solution., Zeeb M, Max KE, Weininger U, Low C, Sticht H, Balbach J, Nucleic Acids Res. 2006;34(16):4561-71. Epub 2006 Sep 6. PMID:16956971

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