1oyu
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1oyu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oyu, resolution 2.5Å" /> '''Long-Distance conform...)
Next diff →
Revision as of 21:11, 20 November 2007
|
Long-Distance conformational changes in a protein engineered by modulated sequence duplication
Overview
There are few, if any, known instances in which a biological signal is, transmitted via a large conformational change through the body of a, protein. We describe here a mutant of T4 lysozyme that was engineered to, permit structural change at a distance. The design uses a tandem sequence, repeat that makes it possible to transmit large-scale structural changes, from one end of an alpha-helix to the other over a distance of 17-25 A., The method should be of general applicability and may make it possible to, introduce a mutation at one site in a protein that will induce large-scale, changes in the structure at a spatially remote site.
About this Structure
1OYU is a Single protein structure of sequence from Bacteriophage t4. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
Reference
Long-distance conformational changes in a protein engineered by modulated sequence duplication., Sagermann M, Gay L, Matthews BW, Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9191-5. Epub 2003 Jul 17. PMID:12869697
Page seeded by OCA on Tue Nov 20 23:18:33 2007
