1svj

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[[Image:1svj.gif|left|200px]]
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{{STRUCTURE_1svj| PDB=1svj | SCENE= }}
{{STRUCTURE_1svj| PDB=1svj | SCENE= }}
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'''The solution structure of the nucleotide binding domain of KdpB'''
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===The solution structure of the nucleotide binding domain of KdpB===
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==Overview==
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P-type ATPases are involved in the active transport of ions across biological membranes. The KdpFABC complex (P-type ATPase) of Escherichia coli is a high-affinity K+ uptake system that operates only when the cell experiences osmotic stress or K+ limitation. Here, we present the solution structure of the nucleotide binding domain of KdpB (backbone RMSD 0.17 A) and a model of the AMP-PNP binding mode based on intermolecular distance restraints. The calculated AMP-PNP binding mode shows the purine ring of the nucleotide to be "clipped" into the binding pocket via a pi-pi-interaction to F377 on one side and a cation-pi-interaction to K395 on the other. This binding mechanism seems to be conserved in all P-type ATPases, except the heavy metal transporting ATPases (type IB). Thus, we conclude that the Kdp-ATPase (currently type IA) is misgrouped and has more similarities to type III ATPases. The KdpB N-domain is the smallest and simplest known for a P-type ATPase, and represents a minimal example of this functional unit. No evidence of significant conformational changes was observed within the N-domain upon nucleotide binding, thus ruling out a role for ATP-induced conformational changes in the reaction cycle.
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{{ABSTRACT_PUBMED_15364580}}
==About this Structure==
==About this Structure==
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1SVJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SVJ OCA].
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1SVJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SVJ OCA].
==Reference==
==Reference==
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[[Category: Kessler, H.]]
[[Category: Kessler, H.]]
[[Category: Alpha-beta sandwich]]
[[Category: Alpha-beta sandwich]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:11:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:34:17 2008''

Revision as of 00:34, 29 July 2008

Image:1svj.png

Template:STRUCTURE 1svj

The solution structure of the nucleotide binding domain of KdpB

Template:ABSTRACT PUBMED 15364580

About this Structure

1SVJ is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

Inter-domain motions of the N-domain of the KdpFABC complex, a P-type ATPase, are not driven by ATP-induced conformational changes., Haupt M, Bramkamp M, Coles M, Altendorf K, Kessler H, J Mol Biol. 2004 Oct 1;342(5):1547-58. PMID:15364580

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