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1svp

From Proteopedia

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{{STRUCTURE_1svp| PDB=1svp | SCENE= }}
{{STRUCTURE_1svp| PDB=1svp | SCENE= }}
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'''SINDBIS VIRUS CAPSID PROTEIN'''
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===SINDBIS VIRUS CAPSID PROTEIN===
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==Overview==
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BACKGROUND: Many enveloped viruses exit cells by budding from the plasma membrane. The driving force for budding is the interaction of an inner protein nucleocapsid core with transmembrane glycoprotein spikes. The molecular details of this process are ill defined. Alphaviruses, such as Sindbis virus (SINV) and Semliki Forest virus (SFV), represent some of the simplest enveloped viruses and have been well characterized by structural, genetic and biochemical techniques. Although a high-resolution structure of an alphavirus has not yet been attained, cryo-electron microscopy (cryo-EM) has been used to show the multilayer organization at 25 A resolution. In addition, atomic resolution studies are available of the C-terminal domain of the nucleocapsid protein and this has been modeled into the cryo-EM density. RESULTS: A recombinant form of Sindbis virus core protein (SCP) was crystallized and found to diffract much better than protein extracted from the virus (2.0 A versus 3.0 A resolution). The new structure showed that amino acids 108 to 111 bind to a specific hydrophobic pocket in neighboring molecules. Re-examination of the structures derived from virus-extracted protein also showed this 'N-terminal arm' binding to the same hydrophobic pocked in adjacent molecules. It is proposed that the binding of these capsid residues into the hydrophobic pocket of SCP mimics the binding of E2 (one of two glycoproteins that penetrate the lipid bilayer of the viral envelope) C-terminal residues in the pocket. Mutational studies of capsid residues 108 and 110 confirm their role in capsid assembly. CONCLUSIONS: Structural and mutational analyses of residues within the hydrophobic pocket suggest that budding results in a switch between two conformations of the capsid hydrophobic pocket. This is the first description of a viral budding mechanism in molecular detail.
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(as it appears on PubMed at http://www.pubmed.gov), where 8736552 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8736552}}
==About this Structure==
==About this Structure==
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[[Category: Mutant]]
[[Category: Mutant]]
[[Category: Sindbis virus capsid protein]]
[[Category: Sindbis virus capsid protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:34:28 2008''

Revision as of 00:34, 29 July 2008

Image:1svp.png

Template:STRUCTURE 1svp

SINDBIS VIRUS CAPSID PROTEIN

Template:ABSTRACT PUBMED 8736552

About this Structure

1SVP is a Single protein structure of sequence from Sindbis virus. Full crystallographic information is available from OCA.

Reference

Identification of a protein binding site on the surface of the alphavirus nucleocapsid and its implication in virus assembly., Lee S, Owen KE, Choi HK, Lee H, Lu G, Wengler G, Brown DT, Rossmann MG, Kuhn RJ, Structure. 1996 May 15;4(5):531-41. PMID:8736552

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