1zkb

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[[Image:1zkb.gif|left|200px]]
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{{Seed}}
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[[Image:1zkb.png|left|200px]]
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{{STRUCTURE_1zkb| PDB=1zkb | SCENE= }}
{{STRUCTURE_1zkb| PDB=1zkb | SCENE= }}
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'''Zinc-free Engineered maltose binding protein'''
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===Zinc-free Engineered maltose binding protein===
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==Overview==
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Protein engineering was used previously to convert maltose-binding protein (MBP) into a zinc biosensor. Zn(2+) binding by the engineered MBP was thought to require a large conformational change from "open" to "closed", similar to that observed when maltose is bound by the wild-type protein. We show that although this re-designed MBP molecule binds Zn(2+) with high affinity as previously reported, it does not adopt a closed conformation in solution as assessed by small-angle X-ray scattering. High-resolution crystallographic studies of the engineered Zn(2+)-binding MBP molecule demonstrate that Zn(2+) is coordinated by residues on the N-terminal lobe only, and therefore Zn(2+) binding does not require the protein to adopt a fully closed conformation. Additional crystallographic studies indicate that this unexpected Zn(2+) binding site can also coordinate Cu(2+) and Ni(2+) with only subtle changes in the overall conformation of the protein. This work illustrates that the energetic barrier to domain closure, which normally functions to maintain MBP in an open concentration in the absence of ligand, is not easily overcome by protein design. A comparison to the mechanism of maltose-induced domain rearrangement is discussed.
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The line below this paragraph, {{ABSTRACT_PUBMED_16288781}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16288781 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16288781}}
==About this Structure==
==About this Structure==
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[[Category: Engineered maltose binding protein]]
[[Category: Engineered maltose binding protein]]
[[Category: Zinc binding mutant]]
[[Category: Zinc binding mutant]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:43:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:36:36 2008''

Revision as of 00:36, 29 July 2008

Image:1zkb.png

Template:STRUCTURE 1zkb

Zinc-free Engineered maltose binding protein

Template:ABSTRACT PUBMED 16288781

About this Structure

1ZKB is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural studies of an engineered zinc biosensor reveal an unanticipated mode of zinc binding., Telmer PG, Shilton BH, J Mol Biol. 2005 Dec 9;354(4):829-40. Epub 2005 Oct 28. PMID:16288781

Page seeded by OCA on Tue Jul 29 03:36:36 2008

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