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1xxi

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{{STRUCTURE_1xxi| PDB=1xxi | SCENE= }}
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'''ADP Bound E. coli Clamp Loader Complex'''
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===ADP Bound E. coli Clamp Loader Complex===
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==Overview==
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Clamp-loader complexes are heteropentameric AAA+ ATPases that load sliding clamps onto DNA. The structure of the nucleotide-free Escherichia coli clamp loader had been determined previously and led to the proposal that the clamp-loader cycles between an inactive state, in which the ATPase domains form a closed ring, and an active state that opens up to form a "C" shape. The crystal structure was interpreted as being closer to the active state than the inactive state. The crystal structure of a nucleotide-bound eukaryotic clamp loader [replication factor C (RFC)] revealed a different and more tightly packed spiral organization of the ATPase domains, raising questions about the significance of the conformation seen earlier for the bacterial clamp loader. We describe crystal structures of the E. coli clamp-loader complex bound to the ATP analog ATPgammaS (at a resolution of 3.5 A) and ADP (at a resolution of 4.1 A). These structures are similar to that of the nucleotide-free clamp-loader complex. Only two of the three functional ATP-binding sites are occupied by ATPgammaS or ADP in these structures, and the bound nucleotides make no interfacial contacts in the complex. These results, along with data from isothermal titration calorimetry, molecular dynamics simulations, and comparison with the RFC structure, suggest that the more open form of the E. coli clamp loader described earlier and in the present work corresponds to a stable inactive state of the clamp loader in which the ATPase domains are prevented from engaging the clamp in the highly cooperative manner seen in the fully ATP-loaded RFC-clamp structure.
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{{ABSTRACT_PUBMED_15556993}}
==About this Structure==
==About this Structure==
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[[Category: Dna polymerase iii]]
[[Category: Dna polymerase iii]]
[[Category: Dna replication]]
[[Category: Dna replication]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:37:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:38:28 2008''

Revision as of 00:38, 29 July 2008

Image:1xxi.png

Template:STRUCTURE 1xxi

ADP Bound E. coli Clamp Loader Complex

Template:ABSTRACT PUBMED 15556993

About this Structure

1XXI is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural analysis of the inactive state of the Escherichia coli DNA polymerase clamp-loader complex., Kazmirski SL, Podobnik M, Weitze TF, O'Donnell M, Kuriyan J, Proc Natl Acad Sci U S A. 2004 Nov 30;101(48):16750-5. Epub 2004 Nov 19. PMID:15556993

Page seeded by OCA on Tue Jul 29 03:38:28 2008

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