2o4a

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2o4a.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2o4a.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2o4a| PDB=2o4a | SCENE= }}
{{STRUCTURE_2o4a| PDB=2o4a | SCENE= }}
-
'''Crystal Structure of the N-terminal CUT Domain of SATB1 Bound to Matrix Attachment Region DNA'''
+
===Crystal Structure of the N-terminal CUT Domain of SATB1 Bound to Matrix Attachment Region DNA===
-
==Overview==
+
<!--
-
Special AT-rich sequence binding protein 1 (SATB1) regulates gene expression essential in immune T-cell maturation and switching of fetal globin species, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin remodeling. Previously we have revealed a five-helix structure of the N-terminal CUT domain, which is essentially the folded region in the MAR-binding domain, of human SATB1 by NMR. Here we determined crystal structure of the complex of the CUT domain and a MAR DNA, in which the third helix of the CUT domain deeply enters the major groove of DNA in the B-form. Bases of 5'-CTAATA-3' sequence are contacted by this helix, through direct and water-mediated hydrogen bonds and apolar and van der Waals contacts. Mutations at conserved base-contacting residues, Gln402 and Gly403, reduced the DNA-binding activity, which confirmed the importance of the observed interactions involving these residues. A significant number of equivalent contacts are observed also for typically four-helix POU-specific domains of POU-homologous proteins, indicating that these domains share a common framework of the DNA-binding mode, recognizing partially similar DNA sequences.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17652321}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17652321 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17652321}}
==About this Structure==
==About this Structure==
Line 28: Line 32:
[[Category: Transcription]]
[[Category: Transcription]]
[[Category: Transcription/dna complex]]
[[Category: Transcription/dna complex]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:18:29 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:40:57 2008''

Revision as of 00:41, 29 July 2008

Image:2o4a.png

Template:STRUCTURE 2o4a

Crystal Structure of the N-terminal CUT Domain of SATB1 Bound to Matrix Attachment Region DNA

Template:ABSTRACT PUBMED 17652321

About this Structure

2O4A is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis for recognition of the matrix attachment region of DNA by transcription factor SATB1., Yamasaki K, Akiba T, Yamasaki T, Harata K, Nucleic Acids Res. 2007;35(15):5073-84. Epub 2007 Jul 25. PMID:17652321

Page seeded by OCA on Tue Jul 29 03:40:57 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2o4a"
Personal tools