2pii

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{{STRUCTURE_2pii| PDB=2pii | SCENE= }}
{{STRUCTURE_2pii| PDB=2pii | SCENE= }}
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'''PII, GLNB PRODUCT'''
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===PII, GLNB PRODUCT===
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==Overview==
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The structure of the bacterial signal transduction protein P(II) has been refined to an R factor of 13.2% using 3sigma data between 10 and 1.9 A. The crystals exhibited twinning by merohedry and X-ray intensities were corrected using the method of Fisher &amp; Sweet [Fisher &amp; Sweet (1980). Acta Cryst. A36, 755-760] prior to refinement. Our earlier 2.7 A structure [Cheah, Carr, Suffolk, Vasudevan, Dixon &amp; Ollis (1994). Structure, 2, 981-990] served as a starting model. P(II) is a trimeric molecule, each subunit has a mass of 12.4 kDa and contains 112 amino-acid residues. The refined model includes all 1065 protein atoms per subunit plus 312 water molecules. The high-resolution refinement confirms the correctness of our 2.7 A model, although it leads to a redefinition of the extent of various secondary-structural elements. The monomeric structure of P(II) exhibits an interlocking double betaalphabeta fold. This is a stable fold found in a number of proteins with diverse functions. The association of the protein into a trimer leads to a new structure which we describe in detail. The effects of crystal packing forces are discussed and potential interaction sites with other proteins and effector molecules are identified.
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(as it appears on PubMed at http://www.pubmed.gov), where 15299730 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15299730}}
==About this Structure==
==About this Structure==
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[[Category: Suffolk, P M.]]
[[Category: Suffolk, P M.]]
[[Category: Signal transduction protein]]
[[Category: Signal transduction protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:48:43 2008''

Revision as of 00:48, 29 July 2008

Image:2pii.png

Template:STRUCTURE 2pii

PII, GLNB PRODUCT

Template:ABSTRACT PUBMED 15299730

About this Structure

2PII is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

X-ray structure of the signal transduction protein from Escherichia coli at 1.9 A., Carr PD, Cheah E, Suffolk PM, Vasudevan SG, Dixon NE, Ollis DL, Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):93-104. PMID:15299730

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