2i13

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[[Image:2i13.gif|left|200px]]
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{{Seed}}
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[[Image:2i13.png|left|200px]]
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{{STRUCTURE_2i13| PDB=2i13 | SCENE= }}
{{STRUCTURE_2i13| PDB=2i13 | SCENE= }}
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'''Aart, a six finger zinc finger designed to recognize ANN triplets'''
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===Aart, a six finger zinc finger designed to recognize ANN triplets===
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==Overview==
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Cys2-His2 zinc fingers are one of the most common types of DNA-binding domains. Modifications to zinc-finger binding specificity have recently enabled custom DNA-binding proteins to be designed to a wide array of target sequences. We present here a 1.96 A structure of Aart, a designed six-zinc finger protein, bound to a consensus DNA target site. This is the first structure of a designed protein with six fingers, and was intended to provide insights into the unusual affinity and specificity characteristics of this protein. Most protein-DNA contacts were found to be consistent with expectations, while others were unanticipated or insufficient to explain specificity. Several were unexpectedly mediated by glycerol, water molecules or amino acid-base stacking interactions. These results challenge some conventional concepts of recognition, particularly the finding that triplets containing 5'A, C, or T are typically not specified by direct interaction with the amino acid in position 6 of the recognition helix.
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(as it appears on PubMed at http://www.pubmed.gov), where 16963084 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16963084}}
==About this Structure==
==About this Structure==
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[[Category: Dna binding]]
[[Category: Dna binding]]
[[Category: Zinc finger]]
[[Category: Zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:56:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:51:40 2008''

Revision as of 00:51, 29 July 2008

Image:2i13.png

Template:STRUCTURE 2i13

Aart, a six finger zinc finger designed to recognize ANN triplets

Template:ABSTRACT PUBMED 16963084

About this Structure

2I13 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structure of Aart, a designed six-finger zinc finger peptide, bound to DNA., Segal DJ, Crotty JW, Bhakta MS, Barbas CF 3rd, Horton NC, J Mol Biol. 2006 Oct 20;363(2):405-21. Epub 2006 Aug 11. PMID:16963084

Page seeded by OCA on Tue Jul 29 03:51:40 2008

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