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2hoa

From Proteopedia

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[[Image:2hoa.jpg|left|200px]]
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{{STRUCTURE_2hoa| PDB=2hoa | SCENE= }}
{{STRUCTURE_2hoa| PDB=2hoa | SCENE= }}
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'''STRUCTURE DETERMINATION OF THE ANTP(C39->S) HOMEODOMAIN FROM NUCLEAR MAGNETIC RESONANCE DATA IN SOLUTION USING A NOVEL STRATEGY FOR THE STRUCTURE CALCULATION WITH THE PROGRAMS DIANA, CALIBA, HABAS AND GLOMSA'''
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===STRUCTURE DETERMINATION OF THE ANTP(C39->S) HOMEODOMAIN FROM NUCLEAR MAGNETIC RESONANCE DATA IN SOLUTION USING A NOVEL STRATEGY FOR THE STRUCTURE CALCULATION WITH THE PROGRAMS DIANA, CALIBA, HABAS AND GLOMSA===
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==Overview==
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The structure of a mutant Antennapedia homeodomain, Antp(C39----S), from Drosophila melanogaster was determined using a set of new programs introduced in the accompanying paper. An input dataset of 957 distance constraints and 171 dihedral angle constraints was collected using two-dimensional n.m.r. experiments with the 15N-labeled protein. The resulting high quality structure for Antp(C39----S), with an average root-mean-square deviation of 0.53 A between the backbone atoms of residues 7 to 59 in 20 energy-refined distance geometry structures and the mean structure, is nearly identical to the previously reported structure of the wild-type Antp homeodomain. The only significant difference is in the connection between helices III and IV, which was found to be less kinked than was indicated by the structure determination for Antp. The main emphasis of the presentation in this paper is on a detailed account of the practical use of a novel strategy for the computation of nuclear magnetic resonance structures of proteins with the combined use of the programs DIANA, CALIBA, HABAS and GLOMSA.
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(as it appears on PubMed at http://www.pubmed.gov), where 1671604 is the PubMed ID number.
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{{ABSTRACT_PUBMED_1671604}}
==About this Structure==
==About this Structure==
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2HOA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOA OCA].
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2HOA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOA OCA].
==Reference==
==Reference==
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[[Category: Wuthrich, K.]]
[[Category: Wuthrich, K.]]
[[Category: Dna-binding protein]]
[[Category: Dna-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:30:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:09:51 2008''

Revision as of 01:09, 29 July 2008

Image:2hoa.png

Template:STRUCTURE 2hoa

STRUCTURE DETERMINATION OF THE ANTP(C39->S) HOMEODOMAIN FROM NUCLEAR MAGNETIC RESONANCE DATA IN SOLUTION USING A NOVEL STRATEGY FOR THE STRUCTURE CALCULATION WITH THE PROGRAMS DIANA, CALIBA, HABAS AND GLOMSA

Template:ABSTRACT PUBMED 1671604

About this Structure

2HOA is a Single protein structure of sequence from Drosophila melanogaster. Full experimental information is available from OCA.

Reference

Structure determination of the Antp (C39----S) homeodomain from nuclear magnetic resonance data in solution using a novel strategy for the structure calculation with the programs DIANA, CALIBA, HABAS and GLOMSA., Guntert P, Qian YQ, Otting G, Muller M, Gehring W, Wuthrich K, J Mol Biol. 1991 Feb 5;217(3):531-40. PMID:1671604

Page seeded by OCA on Tue Jul 29 04:09:51 2008

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