3brd

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{{STRUCTURE_3brd| PDB=3brd | SCENE= }}
{{STRUCTURE_3brd| PDB=3brd | SCENE= }}
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'''CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, P212121'''
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===CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, P212121===
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==Overview==
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The Notch pathway is a conserved cell-to-cell signaling mechanism, in which extracellular signals are transduced into transcriptional outputs through the nuclear effector CSL. CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. The RAM domain of NotchIC avidly interacts with CSL; however, its role in assembly of the CSL-NotchIC-Mastermind ternary complex is not understood. Here we provide a comprehensive thermodynamic, structural, and biochemical analysis of the RAM-CSL interaction for components from both mouse and worm. Our binding data shows that RAM and CSL form a high affinity complex in the presence or absence of DNA. Our structural studies reveal a striking distal conformational change in CSL upon RAM binding, which creates a docking site for Mastermind to bind to the complex. Finally, we show that the addition of a RAM peptide in trans facilitates formation of the CSL-NotchIC-Mastermind ternary complex in vitro.
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{{ABSTRACT_PUBMED_18381292}}
==About this Structure==
==About this Structure==
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[[Category: Transcription]]
[[Category: Transcription]]
[[Category: Transmembrane]]
[[Category: Transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:33:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:16:32 2008''

Revision as of 01:16, 29 July 2008

Image:3brd.png

Template:STRUCTURE 3brd

CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, P212121

Template:ABSTRACT PUBMED 18381292

About this Structure

3BRD is a Protein complex structure of sequences from Caenorhabditis elegans. Full crystallographic information is available from OCA.

Reference

RAM induced allostery facilitates assembly of a notch pathway active transcription complex., Friedmann DR, Wilson JJ, Kovall RA, J Biol Chem. 2008 Apr 1;. PMID:18381292

Page seeded by OCA on Tue Jul 29 04:16:32 2008

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