1pp2

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{{STRUCTURE_1pp2| PDB=1pp2 | SCENE= }}
{{STRUCTURE_1pp2| PDB=1pp2 | SCENE= }}
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'''THE REFINED CRYSTAL STRUCTURE OF DIMERIC PHOSPHOLIPASE A2 AT 2.5 ANGSTROMS. ACCESS TO A SHIELDED CATALYTIC CENTER'''
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===THE REFINED CRYSTAL STRUCTURE OF DIMERIC PHOSPHOLIPASE A2 AT 2.5 ANGSTROMS. ACCESS TO A SHIELDED CATALYTIC CENTER===
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==Overview==
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The 2.5-A crystal structure of the calcium-free form of the dimeric venom phospholipase A2 from the Western Diamondback rattlesnake Crotalus atrox, has been refined to an R-factor of 17.8% (I greater than 2 sigma) and acceptable stereochemistry. The molecule is a nearly perfect 2-fold symmetric dimer in which most of the catalytic residues of both subunits face an internal cavity. The restricted access to the putative catalytic sites is especially puzzling as the optimal substrates for this and most other phospholipase A2 are phospholipids condensed in micellar or lamellar aggregates. We point out that substrate access to the internal cavity may be aided by calcium binding which can alter the intersubunit contacts that shield the catalytic network. We also suggest that a system of hydrogen-bonded moieties exists on the surface of the dimer that links the amino terminus to the catalytic system, through an invariant Gln 4 side chain and the backbone of the active center residue, Tyr 73. This hydrogen-bonded network is on a highly accessible surface of the dimer and would appear to contribute to the enzyme's (as opposed to the proenzyme's) special capacity to attack aggregated rather than monomeric substrate.
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(as it appears on PubMed at http://www.pubmed.gov), where 4019493 is the PubMed ID number.
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{{ABSTRACT_PUBMED_4019493}}
==About this Structure==
==About this Structure==
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[[Category: Sigler, P B.]]
[[Category: Sigler, P B.]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:19:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:18:25 2008''

Revision as of 01:18, 29 July 2008

Image:1pp2.png

Template:STRUCTURE 1pp2

THE REFINED CRYSTAL STRUCTURE OF DIMERIC PHOSPHOLIPASE A2 AT 2.5 ANGSTROMS. ACCESS TO A SHIELDED CATALYTIC CENTER

Template:ABSTRACT PUBMED 4019493

About this Structure

1PP2 is a Single protein structure of sequence from Crotalus atrox. Full crystallographic information is available from OCA.

Reference

The refined crystal structure of dimeric phospholipase A2 at 2.5 A. Access to a shielded catalytic center., Brunie S, Bolin J, Gewirth D, Sigler PB, J Biol Chem. 1985 Aug 15;260(17):9742-9. PMID:4019493

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