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| - | [[Image:2pkf.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2pkf| PDB=2pkf | SCENE= }} | | {{STRUCTURE_2pkf| PDB=2pkf | SCENE= }} |
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| - | '''Crystal structure of M tuberculosis Adenosine Kinase (apo)'''
| + | ===Crystal structure of M tuberculosis Adenosine Kinase (apo)=== |
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| - | ==Overview==
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| - | Adenosine kinase (ADK) catalyzes the phosphorylation of adenosine (Ado) to adenosine monophosphate (AMP). It is part of the purine salvage pathway that has been identified only in eukaryotes, with the single exception of Mycobacterium spp. Whereas it is not clear if Mycobacterium tuberculosis (Mtb) ADK is essential, it has been shown that the enzyme can selectively phosphorylate nucleoside analogs to produce products toxic to the cell. We have determined the crystal structure of Mtb ADK unliganded as well as ligand (Ado) bound at 1.5- and 1.9-A resolution, respectively. The structure of the binary complexes with the inhibitor 2-fluoroadenosine (F-Ado) bound and with the adenosine 5'-(beta,gamma-methylene)triphosphate (AMP-PCP) (non-hydrolyzable ATP analog) bound were also solved at 1.9-A resolution. These four structures indicate that Mtb ADK is a dimer formed by an extended beta sheet. The active site of the unliganded ADK is in an open conformation, and upon Ado binding a lid domain of the protein undergoes a large conformation change to close the active site. In the closed conformation, the lid forms direct interactions with the substrate and residues of the active site. Interestingly, AMP-PCP binding alone was not sufficient to produce the closed state of the enzyme. The binding mode of F-Ado was characterized to illustrate the role of additional non-bonding interactions in Mtb ADK compared with human ADK.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_17597075}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17597075 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17597075}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Tbsgc]] | | [[Category: Tbsgc]] |
| | [[Category: Transferase]] | | [[Category: Transferase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:17:48 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:22:50 2008'' |
Revision as of 01:22, 29 July 2008
Template:STRUCTURE 2pkf
Crystal structure of M tuberculosis Adenosine Kinase (apo)
Template:ABSTRACT PUBMED 17597075
About this Structure
2PKF is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.
Reference
High resolution crystal structures of Mycobacterium tuberculosis adenosine kinase: insights into the mechanism and specificity of this novel prokaryotic enzyme., Reddy MC, Palaninathan SK, Shetty ND, Owen JL, Watson MD, Sacchettini JC, J Biol Chem. 2007 Sep 14;282(37):27334-42. Epub 2007 Jun 26. PMID:17597075
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