2drz

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{{STRUCTURE_2drz| PDB=2drz | SCENE= }}
{{STRUCTURE_2drz| PDB=2drz | SCENE= }}
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'''Crystal structure of the earthworm lectin C-terminal domain mutant in complex with lactose'''
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===Crystal structure of the earthworm lectin C-terminal domain mutant in complex with lactose===
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==Overview==
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Sialic acid (Sia) is a typical terminal sugar, which modifies various types of glycoconjugates commonly found in higher animals. Its regulatory roles in diverse biological phenomena are frequently triggered by interaction with Sia-binding lectins. When using natural Sia-binding lectins as probes, however, there have been practical problems concerning their repertoire and availability. Here, we show a rational creation of a Sia-binding lectin based on the strategy 'natural evolution-mimicry', where Sia-binding lectins are engineered by error-prone PCR from a Gal-binding lectin used as a scaffold protein. After selection with fetuin-agarose using a recently reinforced ribosome display system, one of the evolved mutants SRC showed substantial affinity for alpha2-6Sia, which the parental Gal-binding lectin EW29Ch lacked. SRC was found to have additional practical advantages in productivity and in preservation of affinity for Gal. Thus, the developed novel Sia-recognition protein will contribute as useful tools to sialoglycomics.
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The line below this paragraph, {{ABSTRACT_PUBMED_17234683}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17234683 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17234683}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry., Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J, J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17234683 17234683]
Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry., Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J, J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17234683 17234683]
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Crystallization and preliminary X-ray crystallographic studies of the C-terminal domain of galactose-binding lectin EW29 from the earthworm Lumbricus terrestris., Suzuki R, Fujimoto Z, Kuno A, Hirabayashi J, Kasai K, Hasegawa T, Acta Crystallogr D Biol Crystallogr. 2004 Oct;60(Pt 10):1895-6. Epub 2004, Sep 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15388944 15388944]
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Novel galactose-binding proteins in Annelida. Characterization of 29-kDa tandem repeat-type lectins from the earthworm Lumbricus terrestris., Hirabayashi J, Dutta SK, Kasai K, J Biol Chem. 1998 Jun 5;273(23):14450-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9603958 9603958]
[[Category: Lumbricus terrestris]]
[[Category: Lumbricus terrestris]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Sugar binding protein]]
[[Category: Sugar binding protein]]
[[Category: Sugar complex]]
[[Category: Sugar complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 01:04:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:33:03 2008''

Revision as of 01:33, 29 July 2008

Image:2drz.png

Template:STRUCTURE 2drz

Crystal structure of the earthworm lectin C-terminal domain mutant in complex with lactose

Template:ABSTRACT PUBMED 17234683

About this Structure

2DRZ is a Single protein structure of sequence from Lumbricus terrestris. Full crystallographic information is available from OCA.

Reference

Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry., Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J, J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:17234683

Crystallization and preliminary X-ray crystallographic studies of the C-terminal domain of galactose-binding lectin EW29 from the earthworm Lumbricus terrestris., Suzuki R, Fujimoto Z, Kuno A, Hirabayashi J, Kasai K, Hasegawa T, Acta Crystallogr D Biol Crystallogr. 2004 Oct;60(Pt 10):1895-6. Epub 2004, Sep 23. PMID:15388944

Novel galactose-binding proteins in Annelida. Characterization of 29-kDa tandem repeat-type lectins from the earthworm Lumbricus terrestris., Hirabayashi J, Dutta SK, Kasai K, J Biol Chem. 1998 Jun 5;273(23):14450-60. PMID:9603958

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