1q82

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{{STRUCTURE_1q82| PDB=1q82 | SCENE= }}
{{STRUCTURE_1q82| PDB=1q82 | SCENE= }}
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'''Crystal Structure of CC-Puromycin bound to the A-site of the 50S ribosomal subunit'''
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===Crystal Structure of CC-Puromycin bound to the A-site of the 50S ribosomal subunit===
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==Overview==
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The large ribosomal subunit catalyzes peptide bond formation and will do so by using small aminoacyl- and peptidyl-RNA fragments of tRNA. We have refined at 3-A resolution the structures of both A and P site substrate and product analogues, as well as an intermediate analogue, bound to the Haloarcula marismortui 50S ribosomal subunit. A P site substrate, CCA-Phe-caproic acid-biotin, binds equally to both sites, but in the presence of sparsomycin binds only to the P site. The CCA portions of these analogues are bound identically by either the A or P loop of the 23S rRNA. Combining the separate P and A site substrate complexes into one model reveals interactions that may occur when both are present simultaneously. The alpha-NH(2) group of an aminoacylated fragment in the A site forms one hydrogen bond with the N3 of A2486 (2451) and may form a second hydrogen bond either with the 2' OH of the A-76 ribose in the P site or with the 2' OH of A2486 (2451). These interactions position the alpha amino group adjacent to the carbonyl carbon of esterified P site substrate in an orientation suitable for a nucleophilic attack.
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(as it appears on PubMed at http://www.pubmed.gov), where 12185246 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12185246}}
==About this Structure==
==About this Structure==
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[[Category: Ribosome 50]]
[[Category: Ribosome 50]]
[[Category: Rna-rna complex]]
[[Category: Rna-rna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:55:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:34:24 2008''

Revision as of 01:34, 29 July 2008

Image:1q82.png

Template:STRUCTURE 1q82

Crystal Structure of CC-Puromycin bound to the A-site of the 50S ribosomal subunit

Template:ABSTRACT PUBMED 12185246

About this Structure

1Q82 is a Protein complex structure of sequences from Haloarcula marismortui. Full crystallographic information is available from OCA.

Reference

Structural insights into peptide bond formation., Hansen JL, Schmeing TM, Moore PB, Steitz TA, Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11670-5. Epub 2002 Aug 16. PMID:12185246

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