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1p1t

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{{STRUCTURE_1p1t| PDB=1p1t | SCENE= }}
{{STRUCTURE_1p1t| PDB=1p1t | SCENE= }}
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'''NMR Structure of the N-terminal RRM domain of Cleavage stimulation factor 64 KDa subunit'''
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===NMR Structure of the N-terminal RRM domain of Cleavage stimulation factor 64 KDa subunit===
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==Overview==
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Vertebrate polyadenylation sites are identified by the AAUAAA signal and by GU-rich sequences downstream of the cleavage site. These are recognized by a heterotrimeric protein complex (CstF) through its 64 kDa subunit (CstF-64); the strength of this interaction affects the efficiency of poly(A) site utilization. We present the structure of the RNA-binding domain of CstF-64 containing an RNA recognition motif (RRM) augmented by N- and C-terminal helices. The C-terminal helix unfolds upon RNA binding and extends into the hinge domain where interactions with factors responsible for assembly of the polyadenylation complex occur. We propose that this conformational change initiates assembly. Consecutive Us are required for a strong CstF-GU interaction and we show how UU dinucleotides are recognized. Contacts outside the UU pocket fine tune the protein-RNA interaction and provide different affinities for distinct GU-rich elements. The protein-RNA interface remains mobile, most likely a requirement to bind many GU-rich sequences and yet discriminate against other RNAs. The structural distinction between sequences that form stable and unstable complexes provides an operational distinction between weakly and strongly processed poly(A) sites.
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(as it appears on PubMed at http://www.pubmed.gov), where 12773396 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12773396}}
==About this Structure==
==About this Structure==
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1P1T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P1T OCA].
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1P1T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P1T OCA].
==Reference==
==Reference==
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[[Category: N-terminal helix]]
[[Category: N-terminal helix]]
[[Category: Rna recognition motif]]
[[Category: Rna recognition motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:34:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:40:15 2008''

Revision as of 01:40, 29 July 2008

Image:1p1t.png

Template:STRUCTURE 1p1t

NMR Structure of the N-terminal RRM domain of Cleavage stimulation factor 64 KDa subunit

Template:ABSTRACT PUBMED 12773396

About this Structure

1P1T is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Recognition of GU-rich polyadenylation regulatory elements by human CstF-64 protein., Perez Canadillas JM, Varani G, EMBO J. 2003 Jun 2;22(11):2821-30. PMID:12773396

Page seeded by OCA on Tue Jul 29 04:40:15 2008

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