1r4k

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{{STRUCTURE_1r4k| PDB=1r4k | SCENE= }}
{{STRUCTURE_1r4k| PDB=1r4k | SCENE= }}
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'''Solution Structure of the Drosophila Argonaute 1 PAZ Domain'''
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===Solution Structure of the Drosophila Argonaute 1 PAZ Domain===
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==Overview==
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The discovery of RNA-mediated gene-silencing pathways, including RNA interference, highlights a fundamental role of short RNAs in eukaryotic gene regulation and antiviral defence. Members of the Dicer and Argonaute protein families are essential components of these RNA-silencing pathways. Notably, these two families possess an evolutionarily conserved PAZ (Piwi/Argonaute/Zwille) domain whose biochemical function is unknown. Here we report the nuclear magnetic resonance solution structure of the PAZ domain from Drosophila melanogaster Argonaute 1 (Ago1). The structure consists of a left-handed, six-stranded beta-barrel capped at one end by two alpha-helices and wrapped on one side by a distinctive appendage, which comprises a long beta-hairpin and a short alpha-helix. Using structural and biochemical analyses, we demonstrate that the PAZ domain binds a 5-nucleotide RNA with 1:1 stoichiometry. We map the RNA-binding surface to the open face of the beta-barrel, which contains amino acids conserved within the PAZ domain family, and we define the 5'-to-3' orientation of single-stranded RNA bound within that site. Furthermore, we show that PAZ domains from different human Argonaute proteins also bind RNA, establishing a conserved function for this domain.
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{{ABSTRACT_PUBMED_14615802}}
==About this Structure==
==About this Structure==
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1R4K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R4K OCA].
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1R4K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R4K OCA].
==Reference==
==Reference==
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[[Category: Zhou, M M.]]
[[Category: Zhou, M M.]]
[[Category: Beta barrel]]
[[Category: Beta barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:03:57 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:44:19 2008''

Revision as of 01:44, 29 July 2008

Image:1r4k.png

Template:STRUCTURE 1r4k

Solution Structure of the Drosophila Argonaute 1 PAZ Domain

Template:ABSTRACT PUBMED 14615802

About this Structure

1R4K is a Single protein structure of sequence from Drosophila melanogaster. Full experimental information is available from OCA.

Reference

Structure and conserved RNA binding of the PAZ domain., Yan KS, Yan S, Farooq A, Han A, Zeng L, Zhou MM, Nature. 2003 Nov 27;426(6965):468-74. Epub 2003 Nov 16. PMID:14615802

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