2z67

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{{STRUCTURE_2z67| PDB=2z67 | SCENE= }}
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'''Crystal structure of archaeal O-phosphoseryl-tRNA(Sec) selenium transferase (SepSecS)'''
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===Crystal structure of archaeal O-phosphoseryl-tRNA(Sec) selenium transferase (SepSecS)===
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==Overview==
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The micronutrient selenium is present in proteins as selenocysteine (Sec). In eukaryotes and archaea, Sec is formed in a tRNA-dependent conversion of O-phosphoserine (Sep) by O-phosphoseryl-tRNA:selenocysteinyl-tRNA synthase (SepSecS). Here, we present the crystal structure of Methanococcus maripaludis SepSecS complexed with PLP at 2.5 A resolution. SepSecS, a member of the Fold Type I PLP enzyme family, forms an (alpha2)2 homotetramer through its N-terminal extension. The active site lies on the dimer interface with each monomer contributing essential residues. In contrast to other Fold Type I PLP enzymes, Asn247 in SepSecS replaces the conserved Asp in binding the pyridinium nitrogen of PLP. A structural comparison with Escherichia coli selenocysteine lyase allowed construction of a model of Sep binding to the SepSecS catalytic site. Mutations of three conserved active site arginines (Arg72, Arg94, Arg307), protruding from the neighboring subunit, led to loss of in vivo and in vitro activity. The lack of active site cysteines demonstrates that a perselenide is not involved in SepSecS-catalyzed Sec formation; instead, the conserved arginines may facilitate the selenation reaction. Structural phylogeny shows that SepSecS evolved early in the history of PLP enzymes, and indicates that tRNA-dependent Sec formation is a primordial process.
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(as it appears on PubMed at http://www.pubmed.gov), where 18158303 is the PubMed ID number.
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==About this Structure==
==About this Structure==
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[[Category: Transferase]]
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Revision as of 01:45, 29 July 2008

Image:2z67.png

Template:STRUCTURE 2z67

Crystal structure of archaeal O-phosphoseryl-tRNA(Sec) selenium transferase (SepSecS)

Template:ABSTRACT PUBMED 18158303

About this Structure

2Z67 is a Single protein structure of sequence from Methanococcus maripaludis. Full crystallographic information is available from OCA.

Reference

Structural insights into RNA-dependent eukaryal and archaeal selenocysteine formation., Araiso Y, Palioura S, Ishitani R, Sherrer RL, O'Donoghue P, Yuan J, Oshikane H, Domae N, Defranco J, Soll D, Nureki O, Nucleic Acids Res. 2008 Mar;36(4):1187-99. Epub 2007 Dec 23. PMID:18158303

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