1zdl

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{{STRUCTURE_1zdl| PDB=1zdl | SCENE= }}
{{STRUCTURE_1zdl| PDB=1zdl | SCENE= }}
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'''Crystal Structure of Mouse Thioredoxin Reductase Type 2'''
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===Crystal Structure of Mouse Thioredoxin Reductase Type 2===
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==Overview==
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Thioredoxin reductase (TrxR) is an essential enzyme required for the efficient maintenance of the cellular redox homeostasis, particularly in cancer cells that are sensitive to reactive oxygen species. In mammals, distinct isozymes function in the cytosol and mitochondria. Through an intricate mechanism, these enzymes transfer reducing equivalents from NADPH to bound FAD and subsequently to an active-site disulfide. In mammalian TrxRs, the dithiol then reduces a mobile C-terminal selenocysteine-containing tetrapeptide of the opposing subunit of the dimer. Once activated, the C-terminal redox center reduces a disulfide bond within thioredoxin. In this report, we present the structural data on a mitochondrial TrxR, TrxR2 (also known as TR3 and TxnRd2). Mouse TrxR2, in which the essential selenocysteine residue had been replaced with cysteine, was isolated as a FAD-containing holoenzyme and crystallized (2.6 A; R = 22.2%; R(free) = 27.6%). The addition of NADPH to the TrxR2 crystals resulted in a color change, indicating reduction of the active-site disulfide and formation of a species presumed to be the flavin-thiolate charge transfer complex. Examination of the NADP(H)-bound model (3.0 A; R = 24.1%; R(free) = 31.2%) indicates that an active-site tyrosine residue must rotate from its initial position to stack against the nicotinamide ring of NADPH, which is juxtaposed to the isoalloxazine ring of FAD to facilitate hydride transfer. Detailed analysis of the structural data in conjunction with a model of the unusual C-terminal selenenylsulfide suggests molecular details of the reaction mechanism and highlights evolutionary adaptations among reductases.
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The line below this paragraph, {{ABSTRACT_PUBMED_16217027}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16217027 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16217027}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structures of oxidized and reduced mitochondrial thioredoxin reductase provide molecular details of the reaction mechanism., Biterova EI, Turanov AA, Gladyshev VN, Barycki JJ, Proc Natl Acad Sci U S A. 2005 Oct 18;102(42):15018-23. Epub 2005 Oct 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16217027 16217027]
Crystal structures of oxidized and reduced mitochondrial thioredoxin reductase provide molecular details of the reaction mechanism., Biterova EI, Turanov AA, Gladyshev VN, Barycki JJ, Proc Natl Acad Sci U S A. 2005 Oct 18;102(42):15018-23. Epub 2005 Oct 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16217027 16217027]
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Three-dimensional structure of a mammalian thioredoxin reductase: implications for mechanism and evolution of a selenocysteine-dependent enzyme., Sandalova T, Zhong L, Lindqvist Y, Holmgren A, Schneider G, Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9533-8. Epub 2001 Jul 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11481439 11481439]
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Refined structure of glutathione reductase at 1.54 A resolution., Karplus PA, Schulz GE, J Mol Biol. 1987 Jun 5;195(3):701-29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3656429 3656429]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Selenocysteine]]
[[Category: Selenocysteine]]
[[Category: Thioredoxin]]
[[Category: Thioredoxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:29:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:46:51 2008''

Revision as of 01:46, 29 July 2008

Image:1zdl.png

Template:STRUCTURE 1zdl

Crystal Structure of Mouse Thioredoxin Reductase Type 2

Template:ABSTRACT PUBMED 16217027

About this Structure

1ZDL is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Crystal structures of oxidized and reduced mitochondrial thioredoxin reductase provide molecular details of the reaction mechanism., Biterova EI, Turanov AA, Gladyshev VN, Barycki JJ, Proc Natl Acad Sci U S A. 2005 Oct 18;102(42):15018-23. Epub 2005 Oct 10. PMID:16217027

Three-dimensional structure of a mammalian thioredoxin reductase: implications for mechanism and evolution of a selenocysteine-dependent enzyme., Sandalova T, Zhong L, Lindqvist Y, Holmgren A, Schneider G, Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9533-8. Epub 2001 Jul 31. PMID:11481439

Refined structure of glutathione reductase at 1.54 A resolution., Karplus PA, Schulz GE, J Mol Biol. 1987 Jun 5;195(3):701-29. PMID:3656429

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