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2qar

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[[Image:2qar.jpg|left|200px]]
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{{STRUCTURE_2qar| PDB=2qar | SCENE= }}
{{STRUCTURE_2qar| PDB=2qar | SCENE= }}
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'''Structure of the 2TEL crystallization module fused to T4 lysozyme with a helical linker.'''
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===Structure of the 2TEL crystallization module fused to T4 lysozyme with a helical linker.===
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==Overview==
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Obtaining well-diffracting crystals of macromolecules remains a significant barrier to structure determination. Here we propose and test a new approach to crystallization, in which the crystallization target is fused to a polymerizing protein module, so that polymer formation drives crystallization of the target. We test the approach using a polymerization module called 2TEL, which consists of two tandem sterile alpha motif (SAM) domains from the protein translocation Ets leukemia (TEL). The 2TEL module is engineered to polymerize as the pH is lowered, which allows the subtle modulation of polymerization needed for crystal formation. We show that the 2TEL module can drive the crystallization of 11 soluble proteins, including three that resisted prior crystallization attempts. In addition, the 2TEL module crystallizes in the presence of various detergents, suggesting that it might facilitate membrane protein crystallization. The crystal structures of two fusion proteins show that the TELSAM polymer is responsible for the majority of contacts in the crystal lattice. The results suggest that biological polymers could be designed as crystallization modules.
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(as it appears on PubMed at http://www.pubmed.gov), where 17962407 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17962407}}
==About this Structure==
==About this Structure==
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[[Category: Polymer]]
[[Category: Polymer]]
[[Category: Sterile alpha motif]]
[[Category: Sterile alpha motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:38:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:06:08 2008''

Revision as of 02:06, 29 July 2008

Image:2qar.png

Template:STRUCTURE 2qar

Structure of the 2TEL crystallization module fused to T4 lysozyme with a helical linker.

Template:ABSTRACT PUBMED 17962407

About this Structure

2QAR is a Protein complex structure of sequences from Enterobacteria phage t4 and Escherichia coli. Full crystallographic information is available from OCA.

Reference

Polymer-driven crystallization., Nauli S, Farr S, Lee YJ, Kim HY, Faham S, Bowie JU, Protein Sci. 2007 Nov;16(11):2542-51. PMID:17962407

Page seeded by OCA on Tue Jul 29 05:06:08 2008

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