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2fnb

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{{STRUCTURE_2fnb| PDB=2fnb | SCENE= }}
{{STRUCTURE_2fnb| PDB=2fnb | SCENE= }}
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'''NMR STRUCTURE OF THE FIBRONECTIN ED-B DOMAIN, NMR, 20 STRUCTURES'''
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===NMR STRUCTURE OF THE FIBRONECTIN ED-B DOMAIN, NMR, 20 STRUCTURES===
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==Overview==
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BACKGROUND: The process of angiogenesis (i.e. the formation of new blood vessels from pre-existing ones) is fundamental to physiological processes such as reproduction, development and repair, as well as to pathological conditions such as tumor progression, rheumathoid arthritis and ocular disorders. The oncofoetal ED-B domain, a specific marker of angiogenesis, consists of 91 amino acid residues that are inserted by alternative splicing into the fibronectin (FN) molecule. RESULTS: The NMR structure of the ED-B domain is reported and reveals important differences from other FN type III domains. A comparison of the ED-B domain with the crystal structure of a four-domain FN fragment shows the novel features of ED-B to be located in loop regions that are buried at interdomain interfaces, and which therefore largely determine the global shape of the FN molecule. The negatively charged amino acids in this highly acidic protein are uniformly distributed over the molecular surface, with the sole exception of a solvent-exposed hydrophobic patch that represents a potential specific recognition site. Epitope mapping with 82 decapeptides that span the ED-B sequence revealed that three ED-B-specific monoclonal antibodies, which selectively target newly forming blood vessels in tumor-bearing mice, bind to adjacent regions on the ED-B surface. CONCLUSIONS: The NMR structure enables the identification of a large surface area of the ED-B domain that appears to be accessible in vivo, opening up new diagnostic and therapeutic opportunities. Furthermore, the mapping of specific monoclonal antibodies to the three-dimensional structure of the ED-B domain, and their use in angiogenesis inhibition experiments, provides a basis for further investigation of the role of the ED-B domain in the formation of new blood vessels.
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{{ABSTRACT_PUBMED_10196121}}
==About this Structure==
==About this Structure==
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2FNB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FNB OCA].
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2FNB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FNB OCA].
==Reference==
==Reference==
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[[Category: Fibronectin]]
[[Category: Fibronectin]]
[[Category: Typeiii domain]]
[[Category: Typeiii domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:05:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:09:32 2008''

Revision as of 02:09, 29 July 2008

Image:2fnb.png

Template:STRUCTURE 2fnb

NMR STRUCTURE OF THE FIBRONECTIN ED-B DOMAIN, NMR, 20 STRUCTURES

Template:ABSTRACT PUBMED 10196121

About this Structure

2FNB is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

NMR structure of the human oncofoetal fibronectin ED-B domain, a specific marker for angiogenesis., Fattorusso R, Pellecchia M, Viti F, Neri P, Neri D, Wuthrich K, Structure. 1999 Apr 15;7(4):381-90. PMID:10196121

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