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| - | [[Image:2ucz.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:2ucz.png|left|200px]] |
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| | {{STRUCTURE_2ucz| PDB=2ucz | SCENE= }} | | {{STRUCTURE_2ucz| PDB=2ucz | SCENE= }} |
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| - | '''UBIQUITIN CONJUGATING ENZYME (UBC7) FROM SACCHAROMYCES CEREVISIAE'''
| + | ===UBIQUITIN CONJUGATING ENZYME (UBC7) FROM SACCHAROMYCES CEREVISIAE=== |
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| - | ==Overview==
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| - | Ubiquitin-conjugating enzymes are a family of related proteins that participate in the ubiquitination of proteins. Previous studies on the crystal structures of Saccharomyces cerevisiae Ubc4 and Arabidopsis thaliana Ubc1 indicated that the smallest enzymes (class I), which consist entirely of the conserved core domain, share a common tertiary fold. Here we report the three-dimensional structure of the S. cerevisiae class I enzyme encoded by the UBC7 gene. The crystal structure has been solved using molecular replacement techniques and refined by simulated annealing to an R-factor of 0.183 at 2.93 A resolution. Bond lengths and angles in the molecule have root-mean-square deviations from ideal values of 0.016 A and 2.3 degrees, respectively. Ubc7 is an alpha/beta protein with four alpha-helices and a four-stranded antiparallel beta-sheet. With the exception of two regions where extra residues are present, the tertiary folding of Ubc7 is similar to those of the other two enzymes. The ubiquitin-accepting cysteine is located in a cleft between two loops. One of these loops is nonconserved, as this region of the Ubc7 molecule differs from the other two enzymes by having 13 extra residues. There is also a second single amino acid insertion that alters the orientation of the turn between the first two beta-strands. Analysis of the 13 ubiquitin-conjugating enzyme sequences in S. cerevisiae indicates that there may be two other regions where extra residues could be inserted into the common tertiary fold. Both of these other regions exhibit significant deviations in the superposition of the three structures and, like the two insertion regions in Ubc7, may represent hypervariable regions within a common tertiary fold. As ubiquitin-conjugating enzymes interact with different substrates or other accessory proteins in the ubiquitination pathway, these variable surface regions may confer distinct specificity to individual enzymes.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_9048545}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9048545 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9048545}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Ubiquitin conjugation]] | | [[Category: Ubiquitin conjugation]] |
| | [[Category: Yeast]] | | [[Category: Yeast]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:28:07 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:09:58 2008'' |
Revision as of 02:10, 29 July 2008
Template:STRUCTURE 2ucz
UBIQUITIN CONJUGATING ENZYME (UBC7) FROM SACCHAROMYCES CEREVISIAE
Template:ABSTRACT PUBMED 9048545
About this Structure
2UCZ is a Single protein structure of sequence from Saccharomyces cerevisiae. This structure supersedes the now removed PDB entry 1ucz. Full crystallographic information is available from OCA.
Reference
Crystal structure of a class I ubiquitin conjugating enzyme (Ubc7) from Saccharomyces cerevisiae at 2.9 angstroms resolution., Cook WJ, Martin PD, Edwards BF, Yamazaki RK, Chau V, Biochemistry. 1997 Feb 18;36(7):1621-7. PMID:9048545
Page seeded by OCA on Tue Jul 29 05:09:58 2008
