From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| - | [[Image:2og3.jpg|left|200px]] | + | {{Seed}} |
| | + | [[Image:2og3.png|left|200px]] |
| | | | |
| | <!-- | | <!-- |
| Line 9: |
Line 10: |
| | {{STRUCTURE_2og3| PDB=2og3 | SCENE= }} | | {{STRUCTURE_2og3| PDB=2og3 | SCENE= }} |
| | | | |
| - | '''structure of the rna binding domain of n protein from SARS coronavirus in cubic crystal form'''
| + | ===structure of the rna binding domain of n protein from SARS coronavirus in cubic crystal form=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | Conserved among all coronaviruses are four structural proteins: the matrix (M), small envelope (E), and spike (S) proteins that are embedded in the viral membrane and the nucleocapsid phosphoprotein (N), which exists in a ribonucleoprotein complex in the lumen. The N-terminal domain of coronaviral N proteins (N-NTD) provides a scaffold for RNA binding, while the C-terminal domain (N-CTD) mainly acts as oligomerization modules during assembly. The C terminus of the N protein anchors it to the viral membrane by associating with M protein. We characterized the structures of N-NTD from severe acute respiratory syndrome coronavirus (SARS-CoV) in two crystal forms, at 1.17 A (monoclinic) and at 1.85 A (cubic), respectively, resolved by molecular replacement using the homologous avian infectious bronchitis virus (IBV) structure. Flexible loops in the solution structure of SARS-CoV N-NTD are now shown to be well ordered around the beta-sheet core. The functionally important positively charged beta-hairpin protrudes out of the core, is oriented similarly to that in the IBV N-NTD, and is involved in crystal packing in the monoclinic form. In the cubic form, the monomers form trimeric units that stack in a helical array. Comparison of crystal packing of SARS-CoV and IBV N-NTDs suggests a common mode of RNA recognition, but they probably associate differently in vivo during the formation of the ribonucleoprotein complex. Electrostatic potential distribution on the surface of homology models of related coronaviral N-NTDs suggests that they use different modes of both RNA recognition and oligomeric assembly, perhaps explaining why their nucleocapsids have different morphologies.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_17229691}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17229691 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_17229691}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| Line 33: |
Line 37: |
| | [[Category: Rna binding domain]] | | [[Category: Rna binding domain]] |
| | [[Category: Sars coronavirus]] | | [[Category: Sars coronavirus]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:50:22 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:24:28 2008'' |
Revision as of 02:24, 29 July 2008
Template:STRUCTURE 2og3
structure of the rna binding domain of n protein from SARS coronavirus in cubic crystal form
Template:ABSTRACT PUBMED 17229691
About this Structure
2OG3 is a Single protein structure of sequence from Sars coronavirus. Full crystallographic information is available from OCA.
Reference
Ribonucleocapsid formation of severe acute respiratory syndrome coronavirus through molecular action of the N-terminal domain of N protein., Saikatendu KS, Joseph JS, Subramanian V, Neuman BW, Buchmeier MJ, Stevens RC, Kuhn P, J Virol. 2007 Apr;81(8):3913-21. Epub 2007 Jan 17. PMID:17229691
Page seeded by OCA on Tue Jul 29 05:24:28 2008
