2phe

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[[Image:2phe.gif|left|200px]]
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{{STRUCTURE_2phe| PDB=2phe | SCENE= }}
{{STRUCTURE_2phe| PDB=2phe | SCENE= }}
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'''Model for VP16 binding to PC4'''
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===Model for VP16 binding to PC4===
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==Overview==
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Herpes simplex virion protein 16 (VP16) contains two strong activation regions that can independently and cooperatively activate transcription in vivo. We have identified the regions and residues involved in the interaction with the human transcriptional coactivator positive cofactor 4 (PC4) and the general transcription factor TFIIB. NMR and biochemical experiments revealed that both VP16 activation regions are required for the interaction and undergo a conformational transition from random coil to alpha-helix upon binding to its target PC4. The interaction is strongly electrostatically driven and the binding to PC4 is enhanced by the presence of its amino-terminal domain. We propose models for binding of VP16 to the core domains of PC4 and TFIIB that are based on two independent docking approaches using NMR chemical shift changes observed in titration experiments. The models are consistent with results from site-directed mutagenesis and provide an explanation for the contribution of both acidic and hydrophobic residues for transcriptional activation by VP16. Both intrinsically unstructured activation domains are attracted to their interaction partner by electrostatic interactions, and adopt an alpha-helical conformation around the important hydrophobic residues. The models showed multiple distinct binding surfaces upon interaction with various partners, providing an explanation for the promiscuous properties, cooperativity, and the high activity of this activation domain.
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(as it appears on PubMed at http://www.pubmed.gov), where 15654739 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15654739}}
==About this Structure==
==About this Structure==
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2PHE is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Human_herpesvirus_1 Human herpesvirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PHE OCA].
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2PHE is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Human_herpesvirus_1 Human herpesvirus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PHE OCA].
==Reference==
==Reference==
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[[Category: Transcription]]
[[Category: Transcription]]
[[Category: Vp16]]
[[Category: Vp16]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:06:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:39:09 2008''

Revision as of 02:39, 29 July 2008

Image:2phe.png

Template:STRUCTURE 2phe

Model for VP16 binding to PC4

Template:ABSTRACT PUBMED 15654739

About this Structure

2PHE is a Protein complex structure of sequences from Homo sapiens and Human herpesvirus 1. Full experimental information is available from OCA.

Reference

Structural properties of the promiscuous VP16 activation domain., Jonker HR, Wechselberger RW, Boelens R, Folkers GE, Kaptein R, Biochemistry. 2005 Jan 25;44(3):827-39. PMID:15654739

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