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2z1u

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{{STRUCTURE_2z1u| PDB=2z1u | SCENE= }}
{{STRUCTURE_2z1u| PDB=2z1u | SCENE= }}
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'''Crystal Structure of Hydrogenase Maturation Protein HypE in complex with ATP'''
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===Crystal Structure of Hydrogenase Maturation Protein HypE in complex with ATP===
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==Overview==
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The hydrogenase maturation protein HypE serves an essential function in the biosynthesis of the nitrile group, which is subsequently coordinated to Fe as CN(-) ligands in [Ni-Fe] hydrogenase. Here, we present the crystal structures of HypE from Desulfovibrio vulgaris Hildenborough in the presence and in the absence of ATP at a resolution of 2.0 A and 2.6 A, respectively. Comparison of the apo structure with the ATP-bound structure reveals that binding ATP causes an induced-fit movement of the N-terminal portion, but does not entail an overall structural change. The residue Cys341 at the C terminus, whose thiol group is supposed to be carbamoylated before the nitrile group synthesis, is completely buried within the protein and is located in the vicinity of the gamma-phosphate group of the bound ATP. This suggests that the catalytic reaction occurs in this configuration but that a conformational change is required for the carbamoylation of Cys341. A glutamate residue is found close to the thiol group as well, which is suggestive of deprotonation of the carbamoyl group at the beginning of the reactions.
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{{ABSTRACT_PUBMED_17706667}}
==About this Structure==
==About this Structure==
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[[Category: Beta barrel]]
[[Category: Beta barrel]]
[[Category: Lyase]]
[[Category: Lyase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 19:50:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:45:19 2008''

Revision as of 02:45, 29 July 2008

Image:2z1u.png

Template:STRUCTURE 2z1u

Crystal Structure of Hydrogenase Maturation Protein HypE in complex with ATP

Template:ABSTRACT PUBMED 17706667

About this Structure

2Z1U is a Single protein structure of sequence from Desulfovibrio vulgaris. Full crystallographic information is available from OCA.

Reference

Crystal structures of hydrogenase maturation protein HypE in the Apo and ATP-bound forms., Shomura Y, Komori H, Miyabe N, Tomiyama M, Shibata N, Higuchi Y, J Mol Biol. 2007 Sep 28;372(4):1045-54. Epub 2007 Jul 26. PMID:17706667

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