1tv3

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{{STRUCTURE_1tv3| PDB=1tv3 | SCENE= }}
{{STRUCTURE_1tv3| PDB=1tv3 | SCENE= }}
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'''Crystal structure of the N-methyl-hydroxylamine MtmB complex'''
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===Crystal structure of the N-methyl-hydroxylamine MtmB complex===
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==Overview==
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L-pyrrolysine, the 22(nd) genetically encoded amino acid, was previously deduced to be (4R, 5R)-4-substituted-pyrroline-5-carboxylate attached to the epsilon-nitrogen of lysine based on the crystal structure of the M. barkeri monomethylamine methyltransferase (MtmB). To confirm L-pyrrolysine's identity, structures of MtmB have been determined following treatment with hydroxylamine, N-methylhydroxylamine, or dithionite. Analysis of these structures has provided additional support for the presence of the pyrroline ring and, together with previous mass spectroscopy data, has led us to assign the C(4)-substituent to a methyl group. Based on this assignment, synthetic L-pyrrolysine was prepared by chemical methods. Detailed study of this chemically synthesized L-pyrrolysine has allowed us to characterize its physical properties, to study its chemical stability, and to elucidate the role of its C(4) substituent. Future applications of this synthetic L-pyrrolysine include its in vivo incorporation into recombinant proteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 15380192 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15380192}}
==About this Structure==
==About this Structure==
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[[Category: Zhao, G.]]
[[Category: Zhao, G.]]
[[Category: Tim barrel]]
[[Category: Tim barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:24:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:48:04 2008''

Revision as of 02:48, 29 July 2008

Image:1tv3.png

Template:STRUCTURE 1tv3

Crystal structure of the N-methyl-hydroxylamine MtmB complex

Template:ABSTRACT PUBMED 15380192

About this Structure

1TV3 is a Single protein structure of sequence from Methanosarcina barkeri. Full crystallographic information is available from OCA.

Reference

Reactivity and chemical synthesis of L-pyrrolysine- the 22(nd) genetically encoded amino acid., Hao B, Zhao G, Kang PT, Soares JA, Ferguson TK, Gallucci J, Krzycki JA, Chan MK, Chem Biol. 2004 Sep;11(9):1317-24. PMID:15380192

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