1p9z
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(New page: 200px<br /><applet load="1p9z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p9z" /> '''The Solution Structure of Antifungal Peptide...)
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Revision as of 21:30, 20 November 2007
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The Solution Structure of Antifungal Peptide Distinct With a Five-disulfide Motif from Eucommia ulmoides Oliver
Overview
The three-dimensional structure in aqueous solution of Eucommia antifungal, peptide 2 (EAFP2) from Eucommia ulmoides Oliv was determined using (1)H, NMR spectroscopy. EAFP2 is a newly discovered 41-residue peptide distinct, with a five-disulfide cross-linked motif. This peptide exhibits, chitin-binding activity and inhibitory effects on the growth of cell wall, chitin-containing fungi and chitin-free fungi. The structure was, calculated by using torsion angle dynamic simulated annealing with a total, of 614 distance restraints and 16 dihedral restraints derived from NOESY, and DQF-COSY spectra, respectively. The five disulfide bonds were assigned, from preliminary structures using a statistical analysis of intercystinyl, distances. The solution structure of EAFP2 is presented as an ensemble of, 20 conformers with a backbone RMS deviation of 0.65 (+/-0.13) A for the, well-defined Cys3-Cys39 segment. The tertiary structure of EAFP2, represents the first five-disulfide cross-linked structural model of the, plant antifungal peptide. EAFP2 adopts a compact global fold composed of a, 3(10) helix (Cys3-Arg6), an alpha-helix (Gly26-Cys30), and a three-strand, antiparallel beta-sheet (Cys16-Ser18, Tyr22-Gly24, and Arg36-Cys37). The, tertiary structure of EAFP2 shows a chitin-binding domain (residues 11-30), with a hydrophobic face and a characteristic sector formed by the, N-terminal 10 residues and the C-terminal segment cross-linked through the, unique disulfide bond Cys7-Cys37, which brings all four positively charged, residues (Arg6, Arg9, Arg36, and Arg40) onto a cationic face. On the basis, of such a structural feature, the possible structural basis for the, functional properties of EAFP2 is discussed.
About this Structure
1P9Z is a Single protein structure of sequence from Eucommia ulmoides. Full crystallographic information is available from OCA.
Reference
Solution structure of Eucommia antifungal peptide: a novel structural model distinct with a five-disulfide motif., Huang RH, Xiang Y, Tu GZ, Zhang Y, Wang DC, Biochemistry. 2004 May 25;43(20):6005-12. PMID:15147184
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