2pcf

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{{STRUCTURE_2pcf| PDB=2pcf | SCENE= }}
{{STRUCTURE_2pcf| PDB=2pcf | SCENE= }}
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'''THE COMPLEX OF CYTOCHROME F AND PLASTOCYANIN DETERMINED WITH PARAMAGNETIC NMR. BASED ON THE STRUCTURES OF CYTOCHROME F AND PLASTOCYANIN, 10 STRUCTURES'''
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===THE COMPLEX OF CYTOCHROME F AND PLASTOCYANIN DETERMINED WITH PARAMAGNETIC NMR. BASED ON THE STRUCTURES OF CYTOCHROME F AND PLASTOCYANIN, 10 STRUCTURES===
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==Overview==
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BACKGROUND: The reduction of plastocyanin by cytochrome f is part of the chain of photosynthetic electron transfer reactions that links photosystems II and I. The reaction is rapid and is influenced by charged residues on both proteins. Previously determined structures show that the plastocyanin copper and cytochrome f haem redox centres are some distance apart from the relevant charged sidechains, and until now it was unclear how a transient electrostatic complex can be formed that brings the redox centres sufficiently close for a rapid reaction. RESULTS: A new approach was used to determine the structure of the transient complex between cytochrome f and plastocyanin. Diamagnetic chemical shift changes and intermolecular pseudocontact shifts in the NMR spectrum of plastocyanin were used as input in restrained rigid-body molecular dynamics calculations. An ensemble of ten structures was obtained, in which the root mean square deviation of the plastocyanin position relative to cytochrome f is 1.0 A. Electrostatic interaction is maintained at the same time as the hydrophobic side of plastocyanin makes close contact with the haem area, thus providing a short electron transfer pathway (Fe-Cu distance 10.9 A) via residues Tyr1 or Phe4 (cytochrome f) and the copper ligand His87 (plastocyanin). CONCLUSIONS: The combined use of diamagnetic and paramagnetic chemical shift changes makes it possible to obtain detailed information about the structure of a transient complex of redox proteins. The structure suggests that the electrostatic interactions 'guide' the partners into a position that is optimal for electron transfer, and which may be stabilised by short-range interactions.
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{{ABSTRACT_PUBMED_9551554}}
==About this Structure==
==About this Structure==
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2PCF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Brassica_rapa Brassica rapa] and [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCF OCA].
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2PCF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Brassica_rapa Brassica rapa] and [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCF OCA].
==Reference==
==Reference==
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[[Category: Photosynthesis]]
[[Category: Photosynthesis]]
[[Category: Pseudocontact shift]]
[[Category: Pseudocontact shift]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:50:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:02:56 2008''

Revision as of 03:03, 29 July 2008

Image:2pcf.png

Template:STRUCTURE 2pcf

THE COMPLEX OF CYTOCHROME F AND PLASTOCYANIN DETERMINED WITH PARAMAGNETIC NMR. BASED ON THE STRUCTURES OF CYTOCHROME F AND PLASTOCYANIN, 10 STRUCTURES

Template:ABSTRACT PUBMED 9551554

About this Structure

2PCF is a Protein complex structure of sequences from Brassica rapa and Spinacia oleracea. Full experimental information is available from OCA.

Reference

The structure of the complex of plastocyanin and cytochrome f, determined by paramagnetic NMR and restrained rigid-body molecular dynamics., Ubbink M, Ejdeback M, Karlsson BG, Bendall DS, Structure. 1998 Mar 15;6(3):323-35. PMID:9551554

Page seeded by OCA on Tue Jul 29 06:02:56 2008

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