1twx

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{{STRUCTURE_1twx| PDB=1twx | SCENE= }}
{{STRUCTURE_1twx| PDB=1twx | SCENE= }}
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'''Crystal structure of the thrombin mutant D221A/D222K'''
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===Crystal structure of the thrombin mutant D221A/D222K===
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==Overview==
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The thrombin mutant D221A/D222K (ARK) does not bind Na+ and has interesting functional properties intermediate between those of the slow and fast forms of wild type. We solved the X-ray crystal structure of ARK bound at exosite I with a fragment of hirudin at 2.4-A resolution. The structure shows a slight collapse of the 186 and 220 loops into the Na+ binding site due to disruption of the Asp222:Arg187 ion-pair. The backbone O atoms of Arg221a and Lys224 are shifted into conformations that eliminate optimal interaction with Na+. A paucity of solvent molecules in the Na+ binding site is also noted, by analogy to what is seen in the structure of the slow form. These findings reinforce the crucial role of the Asp222:Arg187 ion-pair in stabilizing the fast form of thrombin.
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{{ABSTRACT_PUBMED_15572256}}
==Disease==
==Disease==
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[[Category: Zhang, E.]]
[[Category: Zhang, E.]]
[[Category: Thrombin]]
[[Category: Thrombin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:15:23 2008''

Revision as of 03:15, 29 July 2008

Image:1twx.png

Template:STRUCTURE 1twx

Contents

Crystal structure of the thrombin mutant D221A/D222K

Template:ABSTRACT PUBMED 15572256

Disease

Known disease associated with this structure: Dysprothrombinemia OMIM:[176930], Hyperprothrombinemia OMIM:[176930], Hypoprothrombinemia OMIM:[176930]

About this Structure

1TWX is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of the thrombin mutant D221A/D222K: the Asp222:Arg187 ion-pair stabilizes the fast form., Pineda AO, Zhang E, Guinto ER, Savvides SN, Tulinsky A, Di Cera E, Biophys Chem. 2004 Dec 20;112(2-3):253-6. PMID:15572256

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