1pc0
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(New page: 200px<br /><applet load="1pc0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pc0" /> '''NMR Structure of the Archaeal Homologue of R...)
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Revision as of 21:33, 20 November 2007
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NMR Structure of the Archaeal Homologue of RNase P Protein Rpp29
Overview
A protein component of the Archaeoglobus fulgidus RNase P was expressed in, Escherichia coli, purified, and structurally characterized using, multidimensional NMR methods. The dominant structural feature of this 11, kDa protein is a sheet of six antiparallel beta-strands, wrapped around a, core of conserved hydrophobic amino acids. Amide proton exchange and (15)N, relaxation rate data provide evidence that the first 16 residues of the, protein, located before the start of the first beta-strand, and the last, 24 residues, located past the end of the last beta-strand, are relatively, flexible; this contrasts with the relatively rigid and well-defined, structure of the beta-sheet. Amino acid sequence comparisons among a, diverse set of species indicate that the A. fulgidus protein is homologous, to the human RNase P protein Rpp29, yeast RNase P protein Pop4, and a, known archaeal RNase P protein from Methanobacter thermoautotrophicus;, conserved hydrophobic residues indicate that the homologous protein in, each of these species contains a similar beta-sheet structure. Conserved, surface residues located in the loop connecting strands beta2 and beta3, the loop connecting strands beta4 and beta5, and in the flexible N- and, C-terminal tails are most likely to have specific interactions with the, RNA and other proteins of RNase P. The structural model of an RNase P, protein component provided by the present work provides an essential step, toward eventually understanding the overall architecture of this complex, enzyme and the mechanism by which it performs its functions.
About this Structure
1PC0 is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
Reference
NMR structure of an archaeal homologue of ribonuclease P protein Rpp29., Sidote DJ, Hoffman DW, Biochemistry. 2003 Nov 25;42(46):13541-50. PMID:14622001
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