2vik

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{{STRUCTURE_2vik| PDB=2vik | SCENE= }}
{{STRUCTURE_2vik| PDB=2vik | SCENE= }}
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'''REFINED STRUCTURE OF THE ACTIN-SEVERING DOMAIN VILLIN 14T, DETERMINED BY SOLUTION NMR, MINIMIZED AVERAGE STRUCTURE'''
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===REFINED STRUCTURE OF THE ACTIN-SEVERING DOMAIN VILLIN 14T, DETERMINED BY SOLUTION NMR, MINIMIZED AVERAGE STRUCTURE===
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==Overview==
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Villin 14T is the amino terminal actin monomer binding domain from the actin-severing and bundling protein villin. Its structure has been determined in solution using heteronuclear multidimensional nuclear magnetic resonance (NMR) spectroscopy (Markus MA, Nakayama T, Matsudaira P, Wagner G. 1994. Solution structure of villin 14T, a domain conserved among actin-severing proteins. Protein Science 3:70-81). An additional nuclear Overhauser effect (NOE) spectroscopy data set, acquired using improved gradient techniques, and further detailed analysis of existing data sets, produced an additional 601 NOE restraints for structure calculations. The overall fold does not change significantly with the additional NOE restraints but the definition of the structure is improved, as judged by smaller deviations among an ensemble of calculated structures that adequately satisfy the NMR restraints. Some of the side chains, especially those in the hydrophobic core of the domain, are much more defined. This improvement in the detail of the solution structure of villin 14T makes it interesting to compare the structure with the crystal structure of gelsolin segment 1, which shares 58% sequence identity with villin 14T, in an effort to gain insight into villin 14T's weaker affinity for actin monomers. Villin 14T has smaller side chains at several positions that make hydrophobic contacts with actin in the context of gelsolin segment 1. The structure is also compared with the structure of the related actin-severing domain, severin domain 2.
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(as it appears on PubMed at http://www.pubmed.gov), where 9194180 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9194180}}
==About this Structure==
==About this Structure==
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2VIK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VIK OCA].
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2VIK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VIK OCA].
==Reference==
==Reference==
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[[Category: Capping protein]]
[[Category: Capping protein]]
[[Category: Cytoskeletal protein]]
[[Category: Cytoskeletal protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:52:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:28:54 2008''

Revision as of 03:28, 29 July 2008

Image:2vik.png

Template:STRUCTURE 2vik

REFINED STRUCTURE OF THE ACTIN-SEVERING DOMAIN VILLIN 14T, DETERMINED BY SOLUTION NMR, MINIMIZED AVERAGE STRUCTURE

Template:ABSTRACT PUBMED 9194180

About this Structure

2VIK is a Single protein structure of sequence from Gallus gallus. Full experimental information is available from OCA.

Reference

Refined structure of villin 14T and a detailed comparison with other actin-severing domains., Markus MA, Matsudaira P, Wagner G, Protein Sci. 1997 Jun;6(6):1197-209. PMID:9194180

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