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1pb3

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{{STRUCTURE_1pb3| PDB=1pb3 | SCENE= }}
{{STRUCTURE_1pb3| PDB=1pb3 | SCENE= }}
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'''Sites of binding and orientation in a four location model for protein stereospecificity.'''
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===Sites of binding and orientation in a four location model for protein stereospecificity.===
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==Overview==
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The stereospecificity of the enzyme isocitrate dehydrogenase was examined by steady-state kinetics and x-ray crystallography. The enzyme has the intriguing property that the apoenzyme in the absence of divalent metal showed a selectivity for the inactive l-enantiomer of the substrate isocitrate, whereas the enzyme containing magnesium showed selectivity for the physiologically active d-enantiomer. The hydrogen atom on the C2 carbon that is transferred during the reaction was, in both the d- and l-isocitrate complexes, in an orientation very close to that expected for delivery of a hydride ion to the cosubstrate NADP+. The beta-carboxylate that is eliminated as a CO2 molecule during the reaction occupied the same site on the protein in both the d- and l-isocitrate complexes. In addition, the C3 carbon was in the same protein site in both the d- and l-enantiomers. Only the fourth group, the OH atom, was in a very different position in the apo enzyme and in the metal-containing complexes. A four-location model is necessary to explain the enantiomeric specificity of IDH in contrast to the conventional three-point attachment model. The thermodynamic and kinetic ramifications of this model are explored.
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(as it appears on PubMed at http://www.pubmed.gov), where 10902579 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10902579}}
==About this Structure==
==About this Structure==
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[[Category: Isocitrate dehydrogense,idh]]
[[Category: Isocitrate dehydrogense,idh]]
[[Category: Stereospecificity]]
[[Category: Stereospecificity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:53:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:29:33 2008''

Revision as of 03:29, 29 July 2008

Image:1pb3.png

Template:STRUCTURE 1pb3

Sites of binding and orientation in a four location model for protein stereospecificity.

Template:ABSTRACT PUBMED 10902579

About this Structure

1PB3 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Sites of binding and orientation in a four-location model for protein stereospecificity., Mesecar AD, Koshland DE Jr, IUBMB Life. 2000 May;49(5):457-66. PMID:10902579

Page seeded by OCA on Tue Jul 29 06:29:33 2008

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