1pd2
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(New page: 200px<br /><applet load="1pd2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pd2, resolution 2.3Å" /> '''CRYSTAL STRUCTURE OF ...)
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Revision as of 21:34, 20 November 2007
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CRYSTAL STRUCTURE OF HEMATOPOIETIC PROSTAGLANDIN D SYNTHASE COMPLEX WITH GLUTATHIONE
Overview
Hematopoietic prostaglandin (PG) D synthase is the key enzyme for, production of the D and J series of prostanoids in the immune system and, mast cells. We isolated a cDNA for the rat enzyme, crystallized the, recombinant enzyme, and determined the three-dimensional structure of the, enzyme complexed with glutathione at 2.3 A resolution. The enzyme is the, first member of the sigma class glutathione S-transferase (GST) from, vertebrates and possesses a prominent cleft as the active site, which is, never seen among other members of the GST family. The unique 3-D, architecture of the cleft leads to the putative substrate binding mode and, its catalytic mechanism, responsible for the specific isomerization from, PGH2 to PGD2.
About this Structure
1PD2 is a Single protein structure of sequence from Rattus norvegicus with GTT as ligand. Active as Prostaglandin-D synthase, with EC number 5.3.99.2 Full crystallographic information is available from OCA.
Reference
Cloning and crystal structure of hematopoietic prostaglandin D synthase., Kanaoka Y, Ago H, Inagaki E, Nanayama T, Miyano M, Kikuno R, Fujii Y, Eguchi N, Toh H, Urade Y, Hayaishi O, Cell. 1997 Sep 19;90(6):1085-95. PMID:9323136
Page seeded by OCA on Tue Nov 20 23:42:11 2007
